Identification of eight genes encoding chemokine-like factor superfamily members 1-8 (CKLFSF1-8) by in silico cloning and experimental validation

被引：169

作者：

Han, WL ^{[1
]}

Ding, PG ^{[1
]}

Xu, MX ^{[1
]}

Wang, L ^{[1
]}

Rui, M ^{[1
]}

Shi, S ^{[1
]}

Liu, YN ^{[1
]}

Zheng, Y ^{[1
]}

Chen, YY ^{[1
]}

Yang, T ^{[1
]}

Ma, DL ^{[1
]}

机构：

[1] Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China

来源：

GENOMICS | 2003年 / 81卷 / 06期

关键词：

CKLF; CKLFSF; bioinformatics; gene cluster; chemokine; TM4SF11;

D O I：

10.1016/S0888-7543(03)00095-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

TM4SF11 is only 102 kb from the chemokine gene cluster composed of SCYA22, SCYD1, and SCYA17 on chromosome 16q13. CKLF maps on chromosome 16q22. CKLFs have some characteristics associated with the CCL22/MDC, CX3CL1/fractalkine, CCL17/TARC, and TM4SF proteins. Bioinformatics based on CKLF2 cDNA and protein sequences in combination with experimental validation identified eight novel genes designated chemokine-like factor superfamily members 1-8 (CKLFSF1-8). CKLFSF1-8 form gene clusters; the sequence identities between CKLF2 and CKLFSF1-8 are from 12.5 to 39.7%. Most of the CKLFSFs have alternative RNA splicing forms. CKLFSF1 has a CC motif and higher sequence similarity with chemokines than with any of the other CKLFSFs. CKLFSF8 shares 39.3% amino acid identity with TM4SF11. CKLFSF1 links the CKLFSF family with chemokines, and CKLFSF8 links it with TM4SF. The characteristics of CKLFSF2-7 are intermediate between CKLFSF1 and CKLFSF8. This indicates that CKLFSF represents a novel gene family between the SCY and the TM4SF gene families. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：609 / 617

页数：9

共 16 条

[1] A new class of membrane-bound chemokine with a CX(3)C motif [J].

Bazan, JF ;

Bacon, KB ;

Hardiman, G ;

Wang, W ;

Soo, K ;

Rossi, D ;

Greaves, DR ;

Zlotnik, A ;

Schall, TJ .

NATURE, 1997, 385 (6617) :640-644

[2]

BRIAN CS, 2002, P NATL ACAD SCI USA, V99, P2129

[3] Chemokine regulation of normal and pathologic immune responses [J].

Christopherson, K ;

Hromas, R .

STEM CELLS, 2001, 19 (05) :388-396

[4] Plasmolipin:: genomic structure, chromosomal localization, protein expression pattern, and putative association with Bardet-Biedl syndrome [J].

Hamacher, M ;

Pippirs, U ;

Köhler, A ;

Müller, HW ;

Bosse, F .

MAMMALIAN GENOME, 2001, 12 (12) :933-937

[5] Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity [J].

Han, WL ;

Lou, YX ;

Tang, JM ;

Zhang, YM ;

Chen, YY ;

Li, Y ;

Gu, WF ;

Huang, JQ ;

Gui, LM ;

Tang, Y ;

Li, F ;

Song, QS ;

Di, CH ;

Wang, L ;

Shi, Q ;

Sun, RH ;

Xia, DL ;

Rui, M ;

Tang, J ;

Ma, DL .

BIOCHEMICAL JOURNAL, 2001, 357 (01) :127-135

[6]

LAURA LK, 2001, GENOMICS, V78, P169

[7] IL-17E, a novel proinflammatory ligand for the IL-17 receptor homolog IL-17Rh1 [J].

Lee, J ;

Ho, WH ;

Maruoka, M ;

Corpuz, RT ;

Baldwin, DT ;

Foster, JS ;

Goddard, AD ;

Yansura, DG ;

Vandlen, RL ;

Wood, WI ;

Gurney, AL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (02) :1660-1664

[8] Cloning and characterization of IL-1HY2, a novel interleukin-1 family member [J].

Lin, HS ;

Ho, AS ;

Haley-Vicente, D ;

Zhang, J ;

Bernal-Fussell, J ;

Pace, AM ;

Hansen, D ;

Schweighofer, K ;

Mize, NK ;

Ford, JE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (23) :20597-20602

[9]

MAGYAR JP, 1997, GENE, V189, P69

[10] Human chemokines fractalkine (SCYD1), MDC (SCYA22) and TARC (SCYA17) are clustered on chromosome 16q13 [J].

Nomiyama, H ;

Imai, T ;

Kusuda, J ;

Miura, R ;

Callen, DF ;

Yoshie, O .

CYTOGENETICS AND CELL GENETICS, 1998, 81 (01) :10-11

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