RETRACTED: Long non-coding RNA SLC25A25-AS1 exhibits oncogenic roles in non-small cell lung cancer by regulating the microRNA-195-5p/ITGA2 axis

被引：15
作者：
Chen, Jinqin [1 ]
Gao, Chengpeng [2 ]
Zhu, Wei [1 ]
机构：
[1] Weifang Peoples Hosp, Dept Chest Surg, 151 Guangwen Rd, Weifang 261401, Shandong, Peoples R China
[2] Weifang Peoples Hosp, Dept Resp Med, Weifang 261401, Shandong, Peoples R China
来源：
ONCOLOGY LETTERS | 2021年 / 22卷 / 01期
关键词：
non-coding RNA; competitive endogenous RNA; microRNA; lung cancer; COMPETING ENDOGENOUS RNA; THERAPY RESISTANCE; EXPRESSION; LNCRNA; PROLIFERATION; TUMORIGENESIS; PATHOGENESIS; BIOMARKERS; DIAGNOSIS; MICRORNAS;
D O I：
10.3892/ol.2021.12790
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Long non-coding RNA SLC25A25 antisense RNA 1 (SLC25A25-AS1) exerts antitumour activity in colorectal cancer. The present study investigated whether SLC25A25-AS1 is implicated in the aggressiveness of non-small cell lung cancer (NSCLC) and the possible underlying mechanism. SLC25A25-AS1 expression in NSCLC was determined by reverse transcription-quantitative PCR. The proliferation, apoptosis, migration and invasion of NSCLC cells were tested in vitro through cell counting kit-8 assay, flow cytometry analysis, Transwell migration and invasion assays, followed by in vivo validation using animal experiments. Additionally, the competitive endogenous RNA theory for SLC25A25-AS1, microRNA-195-5p (miR-195-5p) and integrin alpha 2 (ITGA2) was identified using subcellular fractionation, bioinformatics analysis, reverse transcription-quantitative PCR, western blotting, a luciferase assay and RNA immunoprecipitation. As compared with normal lung tissues, increased expression of SLC25A25-AS1 was demonstrated in NSCLC tissues using The Cancer Genome Atlas database.. In addition, SLC25A25-AS1 was overexpressed in both NSCLC tissues and cell lines. High SLC25A25-AS1 expression was markedly associated with shorter overall survival time of patients with NSCLC. SLC25A25-AS1 silencing impeded NSCLC cell proliferation and triggered apoptosis, while restricting cell migration and invasion. Tumour growth in vivo was also impaired by SLC25A25-AS1 silencing. Mechanistically, SLC25A25-AS1 was demonstrated to be an miR-195-5p sponge in NSCLC cells. miR-195-5p mimics decreased ITGA2 expression in NSCLC cells by directly targeting ITGA2, and SLC25A25-AS1 interference decreased ITGA2 expression by sequestering miR-195-5p. Furthermore, the antitumour effects of SLC25A25-AS1 silencing on malignant behaviours were counteracted when ITGA2 was restored or when miR-195-5p was silenced. In summary, by controlling the miR-195-5p/ITGA2 axis, SLC25A25-AS1 served tumour-promoting roles in NSCLC cells. Therefore, the SLC25A25-AS1/miR-195-5p/ITGA2 signalling pathway might be an attractive target for future therapeutic options in NSCLC.
引用
收藏
页数：13
相关论文
共 43 条
[11]   Long non-coding RNAs: From disease code to drug role [J].
Chen, Yuanyuan ;
Li, Zhaojun ;
Chen, Xiaoguang ;
Zhang, Sen .
ACTA PHARMACEUTICA SINICA B, 2021, 11 (02) :340-354
[12]   The biology and management of non-small cell lung cancer [J].
Herbst, Roy S. ;
Morgensztern, Daniel ;
Boshoff, Chris .
NATURE, 2018, 553 (7689) :446-454
[13]   Effects of microRNA regulation on antiangiogenic therapy resistance in non-small cell lung cancer [J].
Hu, Chenxi ;
Hui, Kaiyuan ;
Jiang, Xiaodong .
BIOMEDICINE & PHARMACOTHERAPY, 2020, 131
[14]   The landscape of long noncoding RNAs in the human transcriptome [J].
Iyer, Matthew K. ;
Niknafs, Yashar S. ;
Malik, Rohit ;
Singhal, Udit ;
Sahu, Anirban ;
Hosono, Yasuyuki ;
Barrette, Terrence R. ;
Prensner, John R. ;
Evans, Joseph R. ;
Zhao, Shuang ;
Poliakov, Anton ;
Cao, Xuhong ;
Dhanasekaran, Saravana M. ;
Wu, Yi-Mi ;
Robinson, Dan R. ;
Beer, David G. ;
Feng, Felix Y. ;
Iyer, Hariharan K. ;
Chinnaiyan, Arul M. .
NATURE GENETICS, 2015, 47 (03) :199-+
[15]   Early Events in the Molecular Pathogenesis of Lung Cancer [J].
Kadara, Humam ;
Scheet, Paul ;
Wistuba, Ignacio I. ;
Spira, Avrum E. .
CANCER PREVENTION RESEARCH, 2016, 9 (07) :518-527
[16]   Competing Endogenous RNA Networks in the Epithelial to Mesenchymal Transition in Diffuse-Type of Gastric Cancer [J].
Landeros, Natalia ;
Santoro, Pablo M. ;
Carrasco-Avino, Gonzalo ;
Corvalan, Alejandro H. .
CANCERS, 2020, 12 (10) :1-21
[17]   Decreased expression of LncRNA SLC25A25-AS1 promotes proliferation, chemoresistance, and EMT in colorectal cancer cells [J].
Li, Yuan ;
Huang, Shengkai ;
Li, Yan ;
Zhang, Weilong ;
He, Kun ;
Zhao, Mei ;
Lin, Hong ;
Li, Dongdong ;
Zhang, Honggang ;
Zheng, Zhaoxu ;
Huang, Changzhi .
TUMOR BIOLOGY, 2016, 37 (10) :14205-14215
[18]   MiR-195 suppresses non-small cell lung cancer by targeting CHEK1 [J].
Liu, Ben ;
Qu, Jinli ;
Xu, Fangxiu ;
Guo, Yan ;
Wang, Yu ;
Yu, Herbert ;
Qian, Biyun .
ONCOTARGET, 2015, 6 (11) :9445-9456
[19]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
[20]   miR-195-5p Suppresses Lung Cancer Cell Proliferation, Migration, and Invasion Via FOXK1 [J].
Long, Zhiqiang ;
Wang, Yadong .
TECHNOLOGY IN CANCER RESEARCH & TREATMENT, 2020, 19
12345