Investigating the predictive value of microRNA21 as a biomarker in induced myocardial infarction animal model

Background: Cardiovascular disease (CVD) is an important cause of morbidity and mortality worldwide. The risk factors for CVD can be unmodifiable, such as advancing age, sex, and genetic factors, or modifiable, like smoking, high blood pressure, abnormal sugar, and obesity. Biomarkers that are used clinically to make a diagnosis of myocardial ischemia include: cardiac enzymes like CKMB and troponin, and these can be used in combination with an electrocardiogram to make a diagnosis. Over the past few years, microRNA has also been used to improve the diagnosis of disease. In this study, we have assessed the use of miRNA-21-3p (miR-21-3p) for early and accurate diagnosis of acute myocardial infarction in a rat model.Methods: Twenty male Wistar rats, divided into 2 groups: 10 healthy ones and 10 induced myocardial infarction rats. Blood samples were taken from these two groups. Real-time PCR was used to measure the serum level of miR-21-3p. A ROC curve analysis was used to evaluate the utility of serum miR-21-3p to diagnose myocardial infarction (MI) in the rat model (AUC: 0.97 (0.91 to 1 at 95% CI) with 100% specificity and 88% sensitivity).Result: The mean CK-MB and troponin I levels inthe control group were higher than AMI group significantly (P < 0.001). Serum expression of miR-21 in rats induced myocardial infarction was significantly higher than control group (p < 0.05).Conclusion: Overexpression of miR-21-3p is a particular importance of acute myocardial infarction (AMI) and can have a special place among the other microRNA in this field.