Inhibitory activity and mechanism of calycosin and calycosin-7-O-β-D-glucoside on α-glucosidase: Spectroscopic and molecular docking analyses

被引：21

作者：

Han, Lingling ^{[1
]}

Song, Jiaqi ^{[1
]}

Yan, Chaoqun ^{[1
]}

Wang, Chunqiang ^{[1
,3
]}

Wang, Liwei ^{[1
]}

Li, Wen ^{[1
]}

Du, Yan ^{[1
]}

Li, Qingshan ^{[1
,2
]}

Liang, Taigang ^{[1
,2
]}

机构：

[1] Shanxi Med Univ, Sch Pharmaceut Sci, 56 Xinjian Nan Rd, Taiyuan 030001, Shanxi, Peoples R China

[2] Shanxi Univ Tradit Chinese Med, 121 Univ St, Jinzhong 030619, Shanxi, Peoples R China

[3] Shanxi Med Univ, Dept Crit Care Med, Peoples Republ, Affiliated Hosp 1, 85 South Jiefang Rd, Taiyuan 030001, Shanxi, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2022年 / 118卷

关键词：

Calycosin-7-O-?-D-glucoside; Calycosin; -Glucosidase; Inhibitory activity; Inhibitory mechanism; KINETICS; PHARMACOLOGY; ROOT;

D O I：

10.1016/j.procbio.2022.04.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibition of alpha-glucosidase activity plays a key role in reducing blood glucose level of type II diabetic patients. In study, the inhibitory activity and mechanism of Calycosin (CA) and calycosin-7-O-beta-D-glucoside (CAG), active components from Astragalus membranaceus, against alpha-glucosidase were studied by alpha-glucosidase activity tests, spectral analysis and docking simulation studies. The results suggested that the alpha-glucosidase inhibitory activity of CA (IC50 =39.45 mu M) and CAG (IC50 =174.04 mu M) were superior to acarbose (positive drug, IC50 =471.73 mu M). Fluorescence data indicated that CA and CAG quenched the fluorescence of alpha-glucosidase by spontaneous forming CA-alpha-glucosidase and CAG-alpha-glucosidase complexes. Besides, results obtained from CD and FT-IR analysis showed that the binding of CA or CAG to alpha-glucosidase caused conformational changes of alpha-glucosidase. Docking simulation results further suggested that, compared with CAG, CA had higher affinity for alpha-glucosidase because of its tightly bound to residues in active cavity of alpha-glucosidase. These findings demonstrated the alpha-glucosidase inhibitory activity and mechanism of CA and CAG, moreover, provided the basis for structural modification of AM flavonoids in the treatment of diabetes mellitus.

引用

页码：227 / 235

页数：9

共 52 条

[1] α-Glucosidase and Protein Tyrosine Phosphatase 1B Inhibitory Activity of Plastoquinones from Marine Brown Alga Sargassum serratifolium [J].

Ali, Md. Yousof ;

Kim, Da Hye ;

Seong, Su Hui ;

Kim, Hyeung-Rak ;

Jung, Hyun Ah ;

Choi, Jae Sue .

MARINE DRUGS, 2017, 15 (12)

[2] Molecular interactions of ceftazidime with bovine serum albumin: Spectroscopic, molecular docking, and DFT analyses [J].

Ali, Mohd Sajid ;

Muthukumaran, Jayaraman ;

Al-Lohedan, Hamad A. .

JOURNAL OF MOLECULAR LIQUIDS, 2020, 313

[3]

Bratkov Viktor M, 2016, Pharmacogn Rev, V10, P11, DOI 10.4103/0973-7847.176550

[4] Prevalence of Diagnosed Diabetes in Adults by Diabetes Type - United States, 2016 [J].

Bullard, Kai McKeever ;

Cowie, Catherine C. ;

Lessem, Sarah E. ;

Saydah, Sharon H. ;

Menke, Andy ;

Geiss, Linda S. ;

Orchard, Trevor J. ;

Rolka, Deborah B. ;

Imperatore, Giuseppina .

MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT, 2018, 67 (12) :359-361

[5] Insight into the inactivation mechanism of soybean Bowman-Birk trypsin inhibitor (BBTI) induced by epigallocatechin gallate and epigallocatechin: Fluorescence, thermodynamics and docking studies [J].

Chen, Zhongqin ;

Chen, Yue ;

Xue, Zihan ;

Gao, Xudong ;

Jia, Yanan ;

Wang, Yajie ;

Lu, Yangpeng ;

Zhang, Jianying ;

Zhang, Min ;

Chen, Haixia .

FOOD CHEMISTRY, 2020, 303

[6] Radix Astragali (Astragalus): Latest Advancements and Trends in Chemistry, Analysis, Pharmacology and Pharmacokinetics [J].

Chu, Chu ;

Qi, Lian-Wen ;

Liu, E-Hu ;

Li, Bin ;

Gao, Wen ;

Li, Ping .

CURRENT ORGANIC CHEMISTRY, 2010, 14 (16) :1792-1807

[7] Investigation the interaction between procyanidin dimer and α-glucosidase: Spectroscopic analyses and molecular docking simulation [J].

Dai, Taotao ;

Chen, Jun ;

McClements, David Julian ;

Li, Ti ;

Liu, Chengmei .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 130 :315-322

[8] Calycosin: a Review of its Pharmacological Effects and Application Prospects [J].

Deng, Mao ;

Chen, Huijuan ;

Long, Jiaying ;

Song, Jiawen ;

Xie, Long ;

Li, Xiaofang .

EXPERT REVIEW OF ANTI-INFECTIVE THERAPY, 2021, 19 (07) :911-925

[9] New Insights into the Inhibition Mechanism of Betulinic Acid on α-Glucosidase [J].

Ding, Huafang ;

Wu, Xiaqing ;

Pan, Junhui ;

Hu, Xing ;

Gong, Deming ;

Zhang, Guowen .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 66 (27) :7065-7075

[10] Review of the Botanical Characteristics, Phytochemistry, and Pharmacology of Astragalus membranaceus (Huangqi) [J].

Fu, Juan ;

Wang, Zenghui ;

Huang, Linfang ;

Zheng, Sihao ;

Wang, Dongmei ;

Chen, Shilin ;

Zhang, Haitao ;

Yang, Shihai .

PHYTOTHERAPY RESEARCH, 2014, 28 (09) :1275-1283

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