The transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program

被引：94

作者：

Itoh-Nakadai, Ari ^{[1
,2
]}

Hikota, Reina ^{[1
]}

Muto, Akihiko ^{[1
,2
]}

Kometani, Kohei ^{[3
]}

Watanabe-Matsui, Miki ^{[1
]}

Sato, Yuki ^{[1
]}

Kobayashi, Masahiro ^{[1
]}

Nakamura, Atsushi ^{[1
]}

Miura, Yuichi ^{[1
]}

Yano, Yoko ^{[4
]}

Tashiro, Satoshi ^{[4
]}

Sun, Jiying ^{[4
]}

Ikawa, Tomokatsu ^{[5
]}

Ochiai, Kyoko ^{[1
,2
,6
]}

Kurosaki, Tomohiro ^{[3
,7
]}

Igarashi, Kazuhiko ^{[1
,2
,6
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Dept Biochem, Sendai, Miyagi 980, Japan

[2] Japan Sci & Technol Agcy, CREST, Sendai, Miyagi, Japan

[3] RIKEN, Ctr Integrat Med Sci, Yokohama, Kanagawa, Japan

[4] Hiroshima Univ, Grad Sch Biomed Sci, Res Inst Radiat Biol & Med, Dept Biol Celular, Hiroshima, Japan

[5] RIKEN, Ctr Integrat Med Sci, Lab Immune Regenerat, Yokohama, Kanagawa, Japan

[6] Tohoku Univ, Grad Sch Med, Ctr Regulatory Epigenome & Dis, Sendai, Miyagi 980, Japan

[7] Osaka Univ, WPI Immunol Frontier Res Ctr, Suita, Osaka, Japan

来源：

NATURE IMMUNOLOGY | 2014年 / 15卷 / 12期

基金：

日本学术振兴会;

关键词：

LINEAGE-COMMITMENT; STEM-CELLS; GENE-EXPRESSION; SPI-C; PROTEINS; NETWORK; EBF1; SPECIFICATION; INTERLEUKIN-7; MACROPHAGES;

D O I：

10.1038/ni.3024

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mature lymphoid cells express the transcription repressor Bach2, which imposes regulation on humoral and cellular immunity. Here we found critical roles for Bach2 in the development of cells of the B lineage, commencing from the common lymphoid progenitor (CLP) stage, with Bach1 as an auxiliary. Overexpression of Bach2 in pre-pro-B cells deficient in the transcription factor EBF1 and single-cell analysis of CLPs revealed that Bach2 and Bach1 repressed the expression of genes important for myeloid cells ('myeloid genes'). Bach2 and Bach1 bound to presumptive regulatory regions of the myeloid genes. Bach2(hi) CLPs showed resistance to myeloid differentiation even when cultured under myeloid conditions. Our results suggest that Bach2 functions with Bach1 and EBF1 to promote B cell development by repressing myeloid genes in CLPs.

引用

页码：1171 / 1180

页数：10