Aptamer-based lateral flow assay on-site biosensors

The lateral flow assay (LFA) is a widely used paper-based on-site biosensor that can detect target analytes and obtain test results in several minutes. Generally, antibodies are utilized as the biorecognition molecules in the LFA. However, antibodies selected using an in vivo process not only may risk killing the animal hosts and causing errors between different batches but also their range is restricted by the refrigerated conditions used to store them. To avoid these limitations, aptamers screened by an in vitro process have been studied as biorecognition molecules in LFAs. Based on the sandwich or competitive format, the aptamer-based LFA can accomplish on-site detection of target analytes. Since aptamers have a distinctive ability to undergo conformational changes, the adsorption-desorption format has also been exploited to detect target analytes in aptamer-based LFAs. This paper reviews developments in aptamer-based LFAs in the last three years for the detection of target analytes. Three formats of aptamer-based LFAs, i.e., sandwich, competitive, and adsorption-desorption, are described in detail. Based on these formats, signal amplification strategies and multiplexed detection are discussed in order to provide an overview of aptamer-based LFAs for on-site detection of target analytes. In addition, the potential commercialization and future perspectives of aptamer-based LFAs for rapid detection of SARS-CoV-2 are given to support the COVID-19 pandemic.