Standardization of photometric measurement of sperm concentration from diploid and tetraploid Pacific oysters, Crassostrea gigas (Thunberg)

被引：24
作者：
Dong, QX
Eudeline, B
Huang, C
Tiersch, TR
机构：
[1] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Aquaculture Res Stn, Baton Rouge, LA 70820 USA
[2] Taylor Resources Hatchery, Quilcene, WA USA
来源：
AQUACULTURE RESEARCH | 2005年 / 36卷 / 01期
关键词：
Crassostrea gigas; sperm concentration; tetraploid;
D O I：
10.1111/j.1365-2109.2004.01188.x
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
To provide necessary standardization of procedures for cryopreservation of sperm, a spectrophotomeric method was developed to determine the sperm concentration of diploid and tetraploid Pacific oysters, Crassostrea gigas. Wavelengths of 380, 550, 581 and 780 nm were compared, and 550 and 581 nm were found to be the most sensitive and reliable. A linear relationship between sperm concentration and photometric absorbance was observed for sperm concentrations between 2 x 10(7) and 2 x 10(9) cells mL(-1). The regression equation for the standard curve at 550 nm for sperm of diploid oysters was Y=-8.528+1.165 log X. The equation for sperm of tetraploid oysters was Y=-8.844+1.236 log X. The equation at 581 nm for sperm of diploid oysters was Y=-8.07+1.104 log X. The equation at 581 nm for sperm of tetraploid oysters was Y=-8.331+1.167 log X. Comparisons derived from the standard curves at 581 nm between observed values and the predicted values indicated good agreement for sperm from diploid (coefficient of determination, r(2)=0.983) and tetraploid (r(2)=0.980) oysters.
引用
收藏
页码：86 / 93
页数：8
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