Selective inhibition of cyclooxygenase-2 by C-phycocyanin, a biliprotein from Spirulina platensis

被引:208
作者
Reddy, CM
Bhat, VB
Kiranmai, G
Reddy, MN
Reddanna, P
Madyastha, KM [1 ]
机构
[1] Indian Inst Sci, Dept Organ Chem, Bangalore 560012, Karnataka, India
[2] JNCASR, Chem Biol Unit, Bangalore 560064, Karnataka, India
[3] Univ Hyderabad, Sch Life Sci, Hyderabad 500046, Andhra Pradesh, India
关键词
nonsteroidal anti-inflammatory drugs; phycocyanin; reduced phycocyanin; phycocyanobilin; cyclooxygenase-1; cyclooxygenase-A; inhibition;
D O I
10.1006/bbrc.2000.3725
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report data from two related assay systems (isolated enzyme assays and whole blood assays) that C-phycocyanin a biliprotein from Spirulina platensis is a selective inhibitor of cyclooxygenase-a (COX-2) with a very low IC50 COX-2/IC50 COX-1 ratio (0.04). The extent of inhibition depends on the period of preincubation of phycocyanin with COX-2, but without any effect on the period of preincubation with COX-1. The IC50 value obtained for the inhibition of COX-2 by phycocyanin is much lower (180 nM) as compared to those of celecoxib (255 nM) and rofecoxib (401 nM), the well-known selective COX-2 inhibitors. In the human whole blood assay, phycocyanin very efficiently inhibited COX-2 with an IC50 value of 80 nM. Reduced phycocyanin and phycocyanobilin, the chromophore of phycocyanin are poor inhibitors of COX-2 without COX-2 selectivity. This suggests that apoprotein in phycocyanin plays a key role in the selective inhibition of COX-2. The present study points out that the hepatoprotective, anti-inflammatory, and anti-arthritic properties of phycocyanin reported in the literature may be due, in part, to its selective COX-2 inhibitory property, although its ability to efficiently scavenge free radicals and effectively inhibit lipid peroxidation may also be involved. (C) 2000 Academic Press.
引用
收藏
页码:599 / 603
页数:5
相关论文
共 37 条
[1]   Oxidative injury induced cyclooxygenase activation in experimental hepatotoxicity [J].
Basu, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 254 (03) :764-767
[2]   C-Phycocyanin:: A potent peroxyl radical scavenger in vivo and in vitro [J].
Bhat, VB ;
Madyastha, KM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (01) :20-25
[3]   ISOLATION AND CHARACTERIZATION OF PHYCOCYANINS FROM THE BLUE-GREEN-ALGA SPIRULINA-PLATENSIS [J].
BOUSSIBA, S ;
RICHMOND, AE .
ARCHIVES OF MICROBIOLOGY, 1979, 120 (02) :155-159
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   A human whole blood assay for clinical evaluation of biochemical efficacy of cyclooxygenase inhibitors [J].
Brideau, C ;
Kargman, S ;
Liu, S ;
Dallob, AL ;
Ehrich, EW ;
Rodger, IW ;
Chan, CC .
INFLAMMATION RESEARCH, 1996, 45 (02) :68-74
[6]   MECHANISM OF SELECTIVE-INHIBITION OF THE INDUCIBLE ISOFORM OF PROSTAGLANDIN G/H SYNTHASE [J].
COPELAND, RA ;
WILLIAMS, JM ;
GIANNARAS, J ;
NURNBERG, S ;
COVINGTON, M ;
PINTO, D ;
PICK, S ;
TRZASKOS, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :11202-11206
[7]   PROSTAGLANDIN - INCREASED PRODUCTION BY RENAL CELL-CARCINOMA [J].
CUMMINGS, KB ;
ROBERTSON, RP .
JOURNAL OF UROLOGY, 1977, 118 (05) :720-723
[8]   Towards a molecular understanding of arthritis [J].
Flugge, LA ;
Miller-Deist, LA ;
Petillo, PA .
CHEMISTRY & BIOLOGY, 1999, 6 (06) :R157-R166
[9]  
Fournier DB, 2000, J CELL BIOCHEM, P97
[10]  
FU E, 1979, BIOCHEM J, V179, P1