Plant regeneration of three Opuntia genotypes used as human food

被引：30

作者：

García-Saucedo, PA ^{[1
]}

Valdez-Morales, M ^{[1
]}

Valverde, ME ^{[1
]}

Cruz-Hernandez, A ^{[1
]}

Paredes-López, O ^{[1
]}

机构：

[1] IPN, Ctr Invest & Estud Avanzados, Dept Biotecnol & Bioquim, Unidad Irapuato, Irapuato 36500, Gto, Mexico

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2005年 / 80卷 / 02期

关键词：

acclimatization; axillary buds; cactaceae; edible cladode; micropropagation; shoot induction;

D O I：

10.1007/s11240-004-9158-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA(3). Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 muM BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp.

引用

页码：215 / 219

页数：5

共 14 条

[1] Temperature checks the Red Queen? Resistance and virulence in a fluctuating environment [J].