Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin

被引：111

作者：

Mizuguchi, G ^{[1
]}

Tsukiyama, T ^{[1
]}

Wisniewski, J ^{[1
]}

Wu, C ^{[1
]}

机构：

[1] NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

MOLECULAR CELL | 1997年 / 1卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(00)80015-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Drosophila nucleosome remodeling factor (NURF) is a protein complex of four subunits that assists transcription factor-mediated perturbation of nucleosomes in an ATP-dependent manner. We have investigated the role of NURF in activating transcription from a preassembled chromatin template and have found that NURF is able to facilitate transcription mediated by a GAL4 derivative carrying both a DNA binding and an activator domain. Interestingly, once nucleosome remodeling by the DNA binding factor is accomplished, a high level of NURF activity is not continuously required for recruitment of the general transcriptional machinery and transcription for at least 100 nucleotides. Our results provide direct evidence that NURF is able to assist gene activation in a chromatin context, and identify a stage of NURF dependence early in the process leading to transcriptional initiation.

引用

页码：141 / 150

页数：10

共 70 条

[1]

Armstrong JA, 1996, MOL CELL BIOL, V16, P5634

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