Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography

被引：99
作者：
Li, L
Tsao, R
Liu, ZQ
Liu, SY
Yang, R
Young, JC
Zhu, HH
Deng, ZY
Xie, MY
Fu, ZH
机构：
[1] Agr & Agri Food Canada, Food Res Program, Guelph, ON N1G 5C9, Canada
[2] Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, Changchun 130022, Peoples R China
[3] NanChang Univ, Minist Educ, Key Lab Food Sci, NanChang, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1063卷 / 1-2期
关键词：
Plantago psyllium; acteoside; isoacteoside; phenylethanoid glycoside; HSCCC; LC-APCI-MS; NMR;
D O I：
10.1016/j.chroma.2004.11.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1: 1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments. (C) 2004 Elsevier B.V. All rights reserved.
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页码：161 / 169
页数：9
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