Preparation of Hydroxyapatite Nanoparticles from Natural Teeth

Hydroxyapatite is the most important bio ceramic, due to its structure and chemical composition which is similar to bone and teeth. In this study, hydroxyapatite nanoparticles were prepared from natural teeth in two stages. Initially, the nano HA particles was performed by whole tooth and in the second stage, the tooth was divided into dentine and root and HA nanoparticles were prepared from both portions of teeth. At first, freshly extracted human teeth both (whole tooth and dentine and root) were calcined at 850 degrees C and then, the effects of stirring time, surfactant and different temperatures were investigated. The XRD results confirmed that, the existence of hydroxyapatite phase in all samples. The phosphate bonding groups of PO43- at 1470 and 669 cm(-1) was confirmed by FTIR results. The FESEM results showed that, hydroxyapatite nano particle with the range of 29-46 nm at stirring time of 36 h with the addition of CTAB as a surfactant at 850 degrees C was obtained without agglomeration and good dispersion.