Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1

被引：24

作者：

Makihira, Seicho ^{[1
]}

Shuto, Takahiro ^{[1
]}

Nikawa, Hiroki ^{[1
]}

Okamoto, Keishi ^{[2
]}

Mine, Yuichi ^{[1
]}

Takamoto, Yuko ^{[1
]}

Ohara, Masaru ^{[3
]}

Tsuji, Koichiro ^{[4
]}

机构：

[1] Hiroshima Univ, Div Oral Hlth Sci, Grad Sch Biomed Sci, Minami Ku, Hiroshima 7348553, Japan

[2] Toyo Adv Technol Co LTD, Minami Ku, Hiroshima 7348501, Japan

[3] Hiroshima Univ Hosp, Minami Ku, Hiroshima 7348553, Japan

[4] Two Cell Co LTD, Minami Ku, Hiroshima 7348553, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 04期

基金：

日本学术振兴会;

关键词：

antimicrobial peptide; titanium surface; MC3T3-E1; cells; ADHERENCE; IMPLANTS; PROLIFERATION; PROTEIN;

D O I：

10.3390/ijms11041458

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control-and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control-and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.

引用

页码：1458 / 1470

页数：13

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