Complete genome sequences of infectious spleen and kidney necrosis virus isolated from farmed albino rainbow sharks Epalzeorhynchos frenatum in the United States

被引：6
作者：
Koda, Samantha A. [1 ]
Subramaniam, Kuttichantran [1 ]
Pouder, Deborah B. [2 ]
Yanong, Roy P. [2 ]
Frasca, Salvatore, Jr. [3 ,5 ]
Popov, Vsevolod L. [4 ]
Waltzek, Thomas B. [1 ]
机构：
[1] Univ Florida, Coll Vet Med, Dept Infect Dis & Immunol, Gainesville, FL 32611 USA
[2] Univ Florida, Trop Aquaculture Lab, Sch Forest Fisheries & Geomat Sci, Inst Food & Agr Sci, Ruskin, FL 33570 USA
[3] Univ Florida, Coll Vet Med, Dept Comparat Diagnost & Populat Med, Gainesville, FL 32611 USA
[4] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA
[5] Univ Connecticut, Connecticut Vet Med Diagnost Lab, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA
来源：
VIRUS GENES | 2021年 / 57卷 / 05期
关键词：
Iridovirus; Megalocytivirus; Infectious spleen and kidney necrosis virus; Complete genome sequence; Rainbow shark; FISH; MEGALOCYTIVIRUS; ALIGNMENT; ISKNV;
D O I：
10.1007/s11262-021-01857-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The genus Megalocytivirus includes viruses known to cause significant disease in aquacultured fish stocks. Herein, we report the complete genome sequences of two megalocytiviruses (MCVs) isolated from diseased albino rainbow sharks Epalzeorhynchos frenatum reared on farms in the United States in 2018 and 2019. Histopathological examination revealed typical megalocytivirus microscopic lesions (i.e., basophilic cytoplasmic inclusions) that were most commonly observed in the spleen and kidney. Transmission electron microscopic examination of spleen and kidney tissues from specimens of the 2018 case revealed hexagonally shaped virus particles with a mean diameter of 153 +/- 6 nm (n = 20) from opposite vertices and 131 +/- 5 nm (n = 20) from opposite faces. Two MCV-specific conventional PCR assays confirmed the presence of MCV DNA in the collected samples. Full genome sequencing of both 2018 and 2019 Epalzeorhynchos frenatus iridoviruses (EFIV) was accomplished using a next-generation sequencing approach. Phylogenomic analyses revealed that both EFIV isolates belong to the infectious spleen and kidney necrosis virus (ISKNV) genotype within the genus Megalocytivirus. This study is the first report of ISKNV in albino rainbow sharks.
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页码：448 / 452
页数：5
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