Genetic diversity analysis of Perinereis aibuhitensis based on ISSR and SRAP markers of Chinese coast populations

被引：6

作者：

Liu, Fei ^{[1
,2
,3
,4
]}

Guo, Qiao-sheng ^{[2
]}

Shi, Hong-zhuan ^{[2
]}

Lv, Fu ^{[1
]}

Yu, Ye-bing ^{[1
]}

Lv, Lin-lan ^{[1
]}

Huang, Jin-tian ^{[1
]}

Wang, Ai-ming ^{[1
]}

Liang, Hui-xing ^{[4
]}

机构：

[1] Yancheng Inst Technol, Dept Ocean Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, Yancheng 224051, Peoples R China

[2] Nanjing Agr Univ, Inst Chinese Med Mat, Nanjing 210095, Jiangsu, Peoples R China

[3] Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China

[4] Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224051, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2014年 / 57卷

基金：

中国博士后科学基金;

关键词：

Perinereis aibuhitensis; ISSR; SRAP; Molecular markers; Genetic diversity; NATURAL-POPULATIONS; SHANDONG PENINSULA; DISTANCE; FLOW; GERMPLASM; PCR;

D O I：

10.1016/j.bse.2014.08.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective of this study was to obtain an overview of the genetic relationships within Perinereis aibuhitensis using Inter-Simple Sequence Repeat (ISSR) and Sequence-Related Amplified Polymorphism (SRAP) markers that were derived from related populations residing in the Chinese coasts. The percentage of polymorphic bands, Nei's gene diversity and Shannon's information index revealed a high level of genetic diversity at the species level. The analysis of molecular variance revealed that 81.22% (ISSR) and 76.29% (SRAP) of variability were partitioned among individuals within populations, which indicated the coherent trend by Nei's genetic differentiation (Gst) (0.2568/0.2876). The gene flow number (Nm) was 1.4470/1.2385, which indicated that there was limited gene exchange between populations. The phylogenetic tree of the ten P. aibuhitensis populations was separated into four major clusters using the neighbor-joining (NJ) method. These results provide a simple and useful basis for P. aibuhitensis germplasm research and aquaculture breeding. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：262 / 269

页数：8

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