High-Level Genetic Diversity among Invasive Streptococcus pneumoniae Isolates in Turkey

被引：5

作者：

Guldemir, Dilek ^{[1
]}

Acar, Sumeyra ^{[1
]}

Otgun, Selin Nar ^{[2
]}

Unaldi, Ozlem ^{[1
]}

Gozalan, Aysegul ^{[3
]}

Ertek, Mustafa ^{[4
]}

Durmaz, Riza ^{[1
,5
]}

机构：

[1] Publ Hlth Inst Turkey, Natl Reference Lab Mol Microbiol, Microbiol Reference Labs Dept, Adnan Saygun Cad 55, TR-06100 Ankara, Turkey

[2] Publ Hlth Inst Turkey, Natl Reference Lab Resp Pathogens, Microbiol Reference Labs Dept, Ankara, Turkey

[3] Ataturk Training & Res Hosp, Dept Clin Microbiol, Ankara, Turkey

[4] Erzincan Univ, Dept Clin Microbiol, Fac Med, Erzincan, Turkey

[5] Yildirim Beyazit Univ, Fac Med, Dept Clin Microbiol, Ankara, Turkey

来源：

JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2016年 / 69卷 / 03期

关键词：

CAPSULAR POLYSACCHARIDE; DISEASE; CHILDREN; IDENTIFICATION; EPIDEMIOLOGY; SEROTYPES; VIRULENCE; PATTERNS;

D O I：

10.7883/yoken.JJID.2015.062

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

This study obtained information on the serotypes and molecular typing characteristics of Streptococcus pneumoniae strains causing invasive diseases in Turkey. Sixty-eight S. pneumoniae isolates causing invasive pneumococcal diseases were collected from different regions of Turkey from 2009 to 2011. The isolates were characterized by performing multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and capsular serotyping, and 25 different serotypes were identified. Serotypes 19F, 23F, 1, 14, and 7F were common and accounted for 52.9% of all the serotypes. In addition, 54 different PFGE profiles (pulsotypes) were observed. Twenty-three of the 68 (33.8%) isolates were clustered into 9 pulsotypes. MLST analysis yielded 36 sequence types, of which 12 (33.3%) were novel. A comparison of results with the global pneumococcal MLST database by performing eBURST analysis showed that our strains belonged to 20 different clonal complexes and 5 singletons. In addition, we identified 4 new alleles: 2 gdh, 1 xpt, and 1 ddl. Thus, the results of this study highlighted a high level of diversity among pneumococcal isolates. In addition, the study identified a case of possible capsular switching.

引用

页码：207 / 212

页数：6

共 26 条

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