Wnt5a promotes differentiation of human dental papilla cells

被引：40

作者：

Peng, L. ^{[1
,2
]}

Ren, L. B. ^{[1
]}

Dong, G. ^{[1
]}

Wang, C. L. ^{[1
]}

Xu, P. ^{[1
]}

Ye, L. ^{[1
]}

Zhou, X. D. ^{[1
,2
]}

机构：

[1] Sichuan Univ, W China Hosp Stomatol, Chengdu, Sichuan, Peoples R China

[2] Sichuan Univ, State Key Lab Oral Dis, Chengdu, Sichuan, Peoples R China

来源：

INTERNATIONAL ENDODONTIC JOURNAL | 2010年 / 43卷 / 05期

关键词：

differentiation; human dental papilla cells; mineralization; Wnt5a; TYROSINE KINASE ROR2; ODONTOBLAST DIFFERENTIATION; OSTEOGENIC DIFFERENTIATION; ALKALINE-PHOSPHATASE; MESENCHYMAL CELLS; PROTEIN-KINASE; TOOTH; OSTEONECTIN; EXPRESSION; PROLIFERATION;

D O I：

10.1111/j.1365-2591.2010.01693.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

P>Aim To investigate the role of Wnt5a in the process of differentiation of human dental papilla cells (HDPCs). Methodology Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate the biological role of Wnt5a on the differentiation of HDPCs. The effect of Wnt5a on HDPCs differentiation was determined by ALP activity assay, ALP staining and mineral induction assay. Mineralization-related gene expressions were assessed by RT-PCR. Results Immunostaining revealed Wnt5a expression in the odontoblast layer and dental papilla tissue. Over-expression of Wnt5a by transfecting HDPCs with an Wnt5a-carrying construct increased ALPase activity and the formation of mineralized nodules of HDPCs. RT-PCR analysis showed that the expressions of mineralization-related genes, such as bone sialoprotein, collagen type I, osteonectin, osteopontin (OCN), dentine matrix protein-1 were up-regulated by Wnt5a. Conclusions Wnt5a promoted differentiation of HDPCs.

引用

页码：404 / 412

页数：9

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