A highly stable raw starch digesting ?-amylase from Nile tilapia (Oreochromis niloticus) viscera

被引：5
作者：
Ferreira, Amalia [1 ]
Cahu, Thiago [1 ]
Xu, Jinchuan [2 ,3 ]
Blennow, Andreas [2 ]
Bezerra, Ranilson [1 ]
机构：
[1] Univ Fed Pernambuco, Dept Biochem, Lab Enzymol LABENZ, Recife, PE, Brazil
[2] Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, Copenhagen, Denmark
[3] South China Univ Technol, Sch Food Sci & Engn, Guangzhou, Peoples R China
来源：
FOOD CHEMISTRY | 2021年 / 354卷
关键词：
Starch; Amylase; Digestive enzymes; Fish processing waste; Starch hydrolysis; ALPHA-AMYLASE; BIOCHEMICAL-CHARACTERIZATION; PURIFICATION; HYDROLYSIS; CLONING; RESISTANT; BACTERIUM; STRAIN; ENZYME; GENE;
D O I：
10.1016/j.foodchem.2021.129513
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A raw starch digesting ?-amylase from Nile tilapia (Oreochromis niloticus) intestine was identified. The ?-amylase, AMY-T, had an estimated molecular weight of 60 kDa and purified to near homogeneity. AMY-T showed an apparent KM 4.78 mg/mL and Vmax 0.44 mg/mL/min) towards soluble starch. It was highly stable for 24 h in the pH range 3.0?10.0, and to solvents like methanol, isopropanol, butanol, dimethylformamide, DMSO and ethylether. AMY-T was able to digest different carbohydrates, mainly showing endo-activity. Importantly, AMY-T was catalytically efficient and adsorbing towards raw potato starch at temperature documented for other raw starch digesting ?-amylases. Thin layer and anion exchange chromatography characterization showed that the end products of raw starch hydrolysis were glucose, maltose and maltodextrins, with degree of polymerisation ranging 1?8. Scanning electron microscopy analysis of the AMY-T treated starch granules documented both granular exo- and endo-attack by AMY-T. These catalytic capabilities suggest high potential for AMY-T for industrial use.
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页数：10
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