Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

被引：51

作者：

van Wamel, W. J. B. ^{[1
,5
]}

Manaskova, S. Hansenova ^{[5
]}

Fluit, A. C. ^{[4
]}

Verbrugh, H. ^{[5
]}

de Neeling, A. J. ^{[2
]}

van Duijkeren, E. ^{[3
]}

van Belkum, A. ^{[5
]}

机构：

[1] Univ Med Ctr Rotterdam, Dept Med Microbiol & Infect Dis, NL-3015 CE Rotterdam, Netherlands

[2] Natl Inst Publ Hlth & Environm RIVM, Bilthoven, Netherlands

[3] Univ Utrecht, Utrecht, Netherlands

[4] Univ Med Ctr Utrecht, Utrecht, Netherlands

[5] Univ Med Ctr Rotterdam, Erasmus MC, Rotterdam, Netherlands

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2010年 / 29卷 / 01期

关键词：

Amplify Fragment Length Polymorphism; Amplify Fragment Length Polymorphism Analysis; SCCmec Type; ST398 Strain; Amplify Fragment Length Polymorphism Fragment;

D O I：

10.1007/s10096-009-0816-3

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

引用

页码：119 / 122

页数：4

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