Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography

In this study, a method combining hollow fiber liquid phase micro-extraction (HF-LPME) and ultra. high performance liquid chromatography (UPLC) was developed for the determination of two alkaloids (piperine and piperlonguminine) in Mongolian medicine Mauleri-Dabusi-4 Soup. The parameters affecting the micro-extraction efficiency were evaluated and optimized. The optimum conditions were as follows: polyvinylidence fluoride HF (54% pore size); 10 g/L NaCl; 30 mu L octanol as extraction solvent; 173 r/min stirring rate of extraction; and 128 min extraction time. The extracted drug was detected by UPLC. The calibration curves obtained good linear relationship in the concentration ranges of 100-8 500 and 8.3-5 000 mu g/L for piperine and piperlonguminine, respectively. The enrichment factors of the method for piperine and piperlonguminine were achieved to be 59 and 65. The limits of detection were 2.2 mu g/L for piperine and 2.5 mu g/L for piperlonguminine. The method was successfully applied for the determination of alkaloids in Mongolian medicine Mauleri-Dabusi-4.