Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

被引：18

作者：

Wu, Shanshan ^{[1
]}

Liu, Xiyang ^{[2
]}

Ye, Shenglong ^{[2
]}

Liu, Jianmin ^{[3
]}

Zheng, Wei ^{[4
]}

Dong, Xue ^{[3
]}

Yin, Xiushan ^{[1
,2
,5
,6
,7
,8
,9
]}

机构：

[1] Shenyang Univ Chem Technol, Coll Pharmaceut & Biol Engn, Appl Biol Lab, Shenyang 110142, Peoples R China

[2] Biotech & Biomed Sci Shenyang Co Ltd, Shenyang 110000, Peoples R China

[3] Shenyang Ctr Dis Control & Prevent, Shenyang 110031, Liaoning, Peoples R China

[4] Liaoning Canc Hosp & Inst, 44 Xiaoheyan Rd, Shenyang 110042, Peoples R China

[5] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SciLifeLab, S-17165 Solna, Sweden

[6] Pluri Biotech Co Ltd, Xuzhou 221001, Jiangsu, Peoples R China

[7] Nanog Biotech Co Ltd, Shanghai 200000, Peoples R China

[8] Biotech & Biomed Sci Jiangxi Co Ltd, Ganzhou 341000, Peoples R China

[9] Shenyang Med Coll, Dept Resp & Crit Care Med, Cent Hosp, Shenyang 110024, Peoples R China

来源：

HELIYON | 2021年 / 7卷 / 04期

关键词：

RT-LAMP; Colorimetric detection; SARS-CoV-2; Dye combination;

D O I：

10.1016/j.heliyon.2021.e06886

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-191,2, which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.

引用

页数：4

共 22 条

[1] Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform [J].

Ahn, Su Jeong ;

Baek, Yun Hee ;

Lloren, Khristine Kaith S. ;

Choi, Won-Suk ;

Jeong, Ju Hwan ;

Antigua, Khristine Joy C. ;

Kwon, Hyeok-il ;

Park, Su-Jin ;

Kim, Eun-Ha ;

Kim, Young-il ;

Si, Young-Jae ;

Hong, Seung Bok ;

Shin, Kyeong Seob ;

Chun, Sungkun ;

Choi, Young Ki ;

Song, Min-Suk .

BMC INFECTIOUS DISEASES, 2019, 19 (1)

[2] Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2 [J].

Baek, Yun Hee ;

Um, Jihye ;

Antigua, Khristine Joy C. ;

Park, Ji-Hyun ;

Kim, Yeonjae ;

Oh, Sol ;

Kim, Young-il ;

Choi, Won-Suk ;

Kim, Seong Gyu ;

Jeong, Ju Hwan ;

Chin, Bum Sik ;

Nicolas, Halcyon Dawn G. ;

Ahn, Ji-Young ;

Shin, Kyeong Seob ;

Choi, Young Ki ;

Park, Jun-Sun ;

Song, Min-Suk .

EMERGING MICROBES & INFECTIONS, 2020, 9 (01) :998-1007

[3]

Evans Jr T.C, 2014, CURR PROTOC MOL BIOL, V105

[4] Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution [J].

Ghadessy, FJ ;

Ramsay, N ;

Boudsocq, F ;

Loakes, D ;

Brown, A ;

Iwai, S ;

Vaisman, A ;

Woodgate, R ;

Holliger, P .

NATURE BIOTECHNOLOGY, 2004, 22 (06) :755-759

[5] Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue [J].

Goto, Motoki ;

Honda, Eiichi ;

Ogura, Atsuo ;

Nomoto, Akio ;

Hanaki, Ken-Ichi .

BIOTECHNIQUES, 2009, 46 (03) :167-+

[6] COVID-19 Infection Diagnosis: Potential Impact of Isothermal Amplification Technology to Reduce Community Transmission of SARS-CoV-2 [J].

James, Ameh S. ;

Alawneh, John I. .

DIAGNOSTICS, 2020, 10 (06)

[7] SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP [J].

Klein, Steffen ;

Mueller, Thorsten G. ;

Khalid, Dina ;

Sonntag-Buck, Vera ;

Heuser, Anke-Mareil ;

Glass, Baerbel ;

Meurer, Matthias ;

Morales, Ivonne ;

Schillak, Angelika ;

Freistaedter, Andrew ;

Ambiel, Ina ;

Winter, Sophie L. ;

Zimmermann, Liv ;

Naumoska, Tamara ;

Bubeck, Felix ;

Kirrmaier, Daniel ;

Ullrich, Stephanie ;

Miranda, Isabel Barreto ;

Anders, Simon ;

Grimm, Dirk ;

Schnitzler, Paul ;

Knop, Michael ;

Kraeusslich, Hans-Georg ;

Viet Loan Dao Thi ;

Boerner, Kathleen ;

Chlanda, Petr .

VIRUSES-BASEL, 2020, 12 (08)

[8]

Larremore DB, 2021, SCI ADV, V7, DOI [10.1126/sciadv.abd5393, 10.1101/2020.06.22.20136309]

[9]

Lin Y., 2020, CLIN CHEM

[10] Evolution of a Thermophilic Strand-Displacing Polymerase Using High-Temperature Isothermal Compartmentalized Self-Replication [J].

Milligan, John N. ;

Shroff, Raghav ;

Garry, Daniel J. ;

Ellington, Andrew D. .

BIOCHEMISTRY, 2018, 57 (31) :4607-4619

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