Extractive fermentation of L-lactic acid with immobilized Rhizopus oryzae in a three-phase fluidized bed

被引：15

作者：

Lin, Jianping

Zhou, Maohong

Zhao, Xiaowei

Luo, Shiyi

Lu, Yinghua ^{[1
]}

机构：

[1] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem Engn & Biochem Engn, Xiamen 361005, Fujian, Peoples R China

[2] Zhejiang Univ, Coll Mat Sci & Chem Engn, Hangzhou 310027, Zhejiang, Peoples R China

[3] Wenzhou Univ, Coll Appl Technol, Wenzhou 352035, Zhejiang, Peoples R China

来源：

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION | 2007年 / 46卷 / 05期

关键词：

L-lactic acid; extractive fermentation; Rhyzopus oryzae; immobilization; three-phase fluidized bed;

D O I：

10.1016/j.cep.2006.06.015

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

A three-phase fluidized bed bioreactor was designed to perform the extractive fermentation of L-(+)-lactic acid by Rhizopus oryzae immobilized in calcium alginate beads. In situ extractive fermentation as well as simultaneous extraction with a coupled solvent column was investigated. The solvent selected was TRPO diluted with sulfonated kerosene. The results indicated that the pH value in the aqueous phase was regulated above 3.5 and product inhibition was alleviated. The production rate was as high as 11 g L-lactic acid/h L beads. A mathematical model Of L-lactic acid production based on the mass balance was also suggested to describe the extractive fermentation processes. The model predictions are in good agreement with the experimental data. (c) 2006 Published by Elsevier B.V.

引用

页码：369 / 374

页数：6

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