Emergence of ST147 Klebsiella pneumoniae Producing OXA-204 Carbapenemase in a University Hospital, Tunisia

被引：23

作者：

Grami, Raoudha ^{[1
,2
]}

Mansour, Wejdene ^{[2
,3
]}

Khalifa, Anis Ben Haj ^{[4
]}

Dahmen, Safia ^{[3
]}

Chatre, Pierre ^{[1
]}

Haenni, Marisa ^{[1
]}

Aouni, Mahjoub ^{[2
]}

Madec, Jean-Yves ^{[1
]}

机构：

[1] ANSES Site Lyon, Unite Antibioresistance & Virulence Bacteriennes, Lyon, France

[2] Fac Pharm, Lab Maladies Transmissibles & Subst Biologiqement, Monastir, Tunisia

[3] Hop Univ Sahloul, Lab Microbiol, Unite Rech Resistances Bacteriennes Emergentes Se, Sousse 4054, Tunisia

[4] Hop Tahar Sfar, Lab Microbiol, Mahdia, Tunisia

来源：

MICROBIAL DRUG RESISTANCE | 2016年 / 22卷 / 02期

关键词：

D BETA-LACTAMASE; MULTIPLEX PCR; OXA-48; ENTEROBACTERIACEAE;

D O I：

10.1089/mdr.2014.0278

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum beta-lactamases and carbapenemase genes were detected by PCR and sequencing. Both isolates were multidrug resistant. KP1 was of sequence type (ST) ST101 and exhibited bla(CTX-M-15) and bla(TEM-1) on an untypeable plasmid and bla(OXA-48) on an IncL/M plasmid. KP2 was genetically unrelated to KP1 (ST147) and harbored an IncA/C plasmid carrying bla(CMY-4) and the bla(OXA-48) derivative gene: bla(OXA-204). This study reports the second case worldwide of an OXA-204-producing K. pneumoniae isolate from the same country, however, in a different genetic background.

引用

页码：137 / 140

页数：4

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