Protein half-lives of two subunits of an NMDA receptor-like complex, the 71-kDa glutamate-binding and the 80-kDa CPP-binding protein

被引:7
|
作者
Chen, XY
Ferrington, DA
Bigelow, DJ
Michaelis, EK [1 ]
机构
[1] Univ Kansas, Dept Pharmacol & Toxicol, Lawrence, KS 66045 USA
[2] Univ Kansas, Dept Biochem, Lawrence, KS 66045 USA
[3] Univ Kansas, Ctr Neurobiol & Immunol Res, Lawrence, KS 66045 USA
关键词
D O I
10.1006/bbrc.1997.7780
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We determined the half-lives for two subunits of a complex that functions as a glutamate and N-methyl-D-aspartate (NMDA) receptor-ion channel in synaptic membranes. These two proteins are a 71 kDa glutamate-binding protein (GBP) and an 80 kDa CPP-binding protein (CBP). Seven month-old Fischer 344 rats were injected with L-[C-14] leucine. The radioactivity in the two proteins was determined in a crude synaptosomal membrane fraction obtained from the brains of rats sacrificed from 4 hours to 13 days after the injection. The previously reported data on time-dependent appearance and loss of L-[C-14] leucine radioactivity in the serum (Ferrington et al., 1997, Biochem. Biophys. Res. Commun. 237, 163-165) was used in the present study to estimate the half-lives of GBP and CBP. Theoretical curves best fit the experimental data obtained for the two proteins assuming apparent half-lives of 14 (+/-2.4) and 18 (+/-1.2) hours for CBP and GBP, respectively. (C) 1997 Academic Press.
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收藏
页码:132 / 135
页数:4
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