Effect of the Carbon Source and Plant Growth Regulators (PGRs) in the Induction and Maintenance of an In Vitro Callus Culture of Taraxacum officinale (L) Weber Ex F.H. Wigg

被引:14
作者
Martinez, Maria Eugenia [1 ,2 ]
Jorquera, Lorena [3 ]
Poirrier, Paola [1 ]
Diaz, Katy [4 ]
Chamy, Rolando [1 ]
机构
[1] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Fac Ingn, Ave Brasil 2085, Valparaiso 237463, Chile
[2] Fraunhofer Chile Res Fdn FCR, Ctr Syst Biotechnol CSB, Avea,Condor 844,3rd Floor of, Huechuraba 8580704, Chile
[3] Pontificia Univ Catolica Valparaiso, Escuela Ingn Construcc, Fac Ingn, Ave Brasil 2147, Valparaiso 237463, Chile
[4] Univ Tecn Federico Santa Maria, Dept Quim, Ave Espana 1680, Valparaiso 2390123, Chile
来源
AGRONOMY-BASEL | 2021年 / 11卷 / 06期
关键词
callus; explant; hypocotyl; PGR; callogenesis; Taraxacum; MEDIATED TRANSFORMATION; REGENERATION; TISSUE; EXPLANTS; HORMONES; LIGHT;
D O I
10.3390/agronomy11061181
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Taraxacum officinale (L.) Weber ex F.H. Wigg, commonly known as dandelion, is a cosmopolitan and perennial weed, which has medicinal properties. In vitro propagation methods are widely used on plants that have difficulties in cultivation and, consequently, low extraction yields of active metabolites. Thus, callus culture has been considered to be useful for the accumulation of several metabolites. In this study, we aimed to establish an efficient protocol for callus induction and maintenance of T. officinale, for which explant type, carbon source, light conditions, and nine different combinations of plant growth regulators (PGRs), such as 1-naphthaleneacetic acid (NAA) (from 0.05 to 0.5 mg/L) and 6-benzylaminopurine acid (BAP) (from 0.5 to 3.0 mg/L), were evaluated. The results showed that hypocotyls and roots from sterile seedlings are the best sources for callus induction, with 100% of callogenesis at every condition tested, and more than 95% of viability and friability. Complete darkness and a medium supplemented with sucrose at 2.3% (w/v) and 0.5 mg/L of NAA and 0.5 mg/L of BAP were the best conditions for callus induction, showing callus with low organogenesis and high friability. This study provides a basis for future studies on improving large-scale callus propagation and further establishment of suspension culture systems for commercial purposes.
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页数:17
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