Metabolomic profiles of A-type procyanidin dimer and trimer with gut microbiota in vitro

被引:20
作者
Chen, Wanbing [1 ,2 ]
Zhang, Li [4 ]
Zhao, Li [1 ,2 ]
Yan, Fangfang [1 ,2 ]
Zhu, Xiaoling [4 ]
Lu, Qun [1 ,2 ,3 ]
Liu, Rui [1 ,2 ,3 ]
机构
[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
[2] Wuhan Engn Res Ctr Bee Prod Qual & Safety Control, Wuhan 430070, Peoples R China
[3] Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China
[4] Hubei Prov Inst Food Supervis & Test, Wuhan 430070, Peoples R China
关键词
A-type procyanidins; Metabolism; Gut microbiota; Metabolomics; (-)-EPICATECHIN; CATABOLISM; ACID; CELL; B2;
D O I
10.1016/j.jff.2021.104637
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Although intestinal microbiota plays an important role in the metabolism of procyanidins, the microbial metabolic pathway of A-type procyanidins remains elusive. In this study, A-type dimer procyanidin A1 [EC-(28-O7,48-8)-C] and trimer PPD [EC-(48-6)-EC-(28-O-7,48-8)-C] were incubated with rat faecal microbiota in vitro, and their metabolomic profiles were analyzed by UPLC-QTOF/MS. Multivariate statistical analysis identified 24 and 30 discriminant metabolites for A1 and PPD, respectively. The C-ring opened catabolites of procyanidin A1 and PPD and the typical phenolic acid metabolites were identified, including phenylacetic acid, (4 '-hydroxyphenyl)acetic acid, 3-phenylpropanoic acid, 3-(4 '-hydroxyphenyl)propanoic acid, 3-(3 ',4 '-dihydroxyphenyl) propanoic acid, and 5-(3 ',4 '-dihydroxyphenyl)-gamma-valerolactone. Notably, procyanidin A1 and (epi)catechin were identified from the metabolites of PPD; however, no (epi)catechin was found in the metabolites of procyanidin A1, indicating that the faecal microbiota can destroy B-type linkage (C4-C6 interflavan bond) but not A-type linkage (C4-C8 and additional C2-O-C7 interflavan bond) in the metabolism of A-type procyanidins.
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页数:11
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