Properties and development of calcium currents in embryonic cockroach neurons

被引：7

作者：

Benquet, P ^{[1
]}

Frere, S ^{[1
]}

Pichon, Y ^{[1
]}

Tiaho, F ^{[1
]}

机构：

[1] Equipe Canaux & Recepteurs Membranaires, UMR 6026, F-35042 Rennes, France

来源：

NEUROSCIENCE LETTERS | 2000年 / 294卷 / 01期

关键词：

calcium channels; development; embryonic neurons; patch-clamp; insect; toxin; calcium channel blockers;

D O I：

10.1016/S0304-3940(00)01549-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In freshly dissociated neurons from embryonic cockroach (Periplaneta americana L.) brains, voltage-dependent calcium currents appear early in development (E14). Their intensity increases progressively during embryonic life until eclosion (E35). Their time course and voltage dependency are characteristic of high voltage activated (HVA) currents although a 10 mV shift of the I/V curve towards more negative potentials was observed between E18 and E23. Their sensitivity to omega -AgaTx-IVA and omega -CgTx-GVIA and insensitivity to both amiloride and isradipine indicate that the corresponding channels are of the P/Q and N types. These channels, as well as a small proportion of toxin-resistant (R) channels (about 20%), are blocked by mibefradil and verapamil. The physiological significance of these currents and their modifications during embryonic life is discussed. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：49 / 52

页数：4

共 23 条

[1]

Baines RA, 1998, J NEUROSCI, V18, P4673

[2] ω-AgaIVA-sensitive (P/Q-type) and -resistant (R-type) high-voltage-activated Ba2+ currents in embryonic cockroach brain neurons [J].

Benquet, P ;

Le Guen, J ;

Dayanithi, G ;

Pichon, Y ;

Tiaho, F .

JOURNAL OF NEUROPHYSIOLOGY, 1999, 82 (05) :2284-2293

[3]

BYERLY L, 1988, J NEUROSCI, V8, P4379

[4] CALCIUM AND POTASSIUM CURRENTS IN THE FAST COXAL DEPRESSOR MOTOR-NEURON OF THE COCKROACH PERIPLANETA-AMERICANA [J].

DAVID, JA ;

PITMAN, RM .

JOURNAL OF NEUROPHYSIOLOGY, 1995, 74 (05) :2043-2050

[5] INVIVO DEVELOPMENT OF VOLTAGE-DEPENDENT IONIC CURRENTS IN EMBRYONIC XENOPUS SPINAL NEURONS [J].

DESARMENIEN, MG ;

CLENDENING, B ;

SPITZER, NC .

JOURNAL OF NEUROSCIENCE, 1993, 13 (06) :2575-2581

[6] Developmental regulation of T-, N- and L-type calcium currents in mouse embryonic sensory neurones [J].

Desmadryl, G ;

Hilaire, C ;

Vigues, S ;

Diochot, S ;

Valmier, J .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1998, 10 (02) :545-552

[7] DIVERSITY OF VOLTAGE-GATED CALCIUM CURRENTS IN LARGE-DIAMETER EMBRYONIC MOUSE SENSORY NEURONS [J].

DIOCHOT, S ;

RICHARD, S ;

VALMIER, J .

NEUROSCIENCE, 1995, 69 (02) :627-641

[8] DIHYDRAPYRIDINES, PHENYLALKYLAMINES AND BENZOTHIAZEPINES BLOCK N-TYPE, P/Q-TYPE AND R-TYPE CALCIUM CURRENTS [J].

DIOCHOT, S ;

RICHARD, S ;

BALDYMOULINIER, M ;

NARGEOT, J ;

VALMIER, J .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1995, 431 (01) :10-19

[9] EMBRYONIC-DEVELOPMENT OF IDENTIFIED NEURONS - DIFFERENTIATION FROM NEUROBLAST TO NEURON [J].

GOODMAN, CS ;

SPITZER, NC .

NATURE, 1979, 280 (5719) :208-214

[10] Breaking the code: Regulation of neuronal differentiation by spontaneous calcium transients [J].

Gu, XN ;

Spitzer, NC .

DEVELOPMENTAL NEUROSCIENCE, 1997, 19 (01) :33-41

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