A heterologous production of the Trametes hirsuta laccase in the fungus Penicillium canescens

被引：12

作者：

Abyanova, A. R. ^{[1
]}

Chulkin, A. M. ^{[1
]}

Vavilova, E. A. ^{[1
]}

Fedorova, T. V. ^{[1
]}

Loginov, D. S. ^{[1
]}

Koroleva, O. V. ^{[1
]}

Benevolensky, S. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Bach Inst Biochem, Moscow, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2010年 / 46卷 / 03期

关键词：

VERSICOLOR LACCASE; NITRATE REDUCTASE; EXPRESSION; GENE; CLONING;

D O I：

10.1134/S0003683810030117

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A heterologous protein expression in the fungus Penicillium canescens is described for the first time. The fungal strains producing Trametes hirsuta 072 accase under control of a highly efficient promoter of the P. canescens gene bgaS has been constructed. These strains efficiently transcribe the T. hirsuta 072 laccase gene with a correct intron splicing. Activity of the secreted heterologous laccase in the culture liquid reaches 3 U/ml, accounting for 98% of the total laccase activity, which demonstrates a high efficiency of heterologous secretion. The synthesized P. canescens laccase has the same molecular weight as the enzyme produced by T. hirsuta 072.

引用

页码：313 / 317

页数：5

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