Antiproliferative activity and induction of apoptosis in human colon cancer cells treated in vitro with constituents of a product derived from Pistacia lentiscus L. var. chia

被引:117
作者
Balan, K. V.
Prince, J.
Han, Z.
Dimas, K.
Cladaras, M.
Wyche, J. H.
Sitaras, N. M.
Pantazis, P. [1 ]
机构
[1] Univ Miami, Dept Biol, Miami, FL 33152 USA
[2] Acad Athens, Fdn Biomed Res, Lab Pharmacol Pharmcotechnol, Athens, Greece
[3] Aristotle Univ Thessaloniki, Sch Biol, GR-54006 Thessaloniki, Greece
[4] Univ Athens, Sch Med, Dept Pharmacol, GR-11527 Athens, Greece
关键词
Chios mastic gum; colon cancer cells; apoptosis; Pistacia lentiscus L. var. chia;
D O I
10.1016/j.phymed.2006.03.009
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In this report, we demonstrate that a 50% ethanol extract of the plant-derived product, Chios mastic gum (CMG), contains compounds which inhibit proliferation and induce death of HCT116 human colon cancer cells in vitro. CMG treatment induces cell arrest at G(1), detachment of the cells from the substrate, activation of pro-caspases-8, -9 and -3, and causes several morphological changes typical of apoptosis in cell organelles. These events, furthermore, are time- and dose-dependent, but p53- and p21-independent. Apoptosis induction by CMG is not inhibited in HCT116 cell clones expressing high levels of the anti-apoptotic protein, Bcl-2, or dominant-negative FADD, thereby indicating that CMG induces cell death via a yet-to-be identified pathway, unrelated to the death receptor- and mitochondrion-dependent pathways. The findings presented here suggest that CMG (a) induces an anoikis form of cell death in HCT116 colon cancer cells that includes events associated with caspase-dependent pathways; and (b) might be developed into a chemotherapeutic agent for the treatment of human colon and other cancers. (c) 2006 Elsevier GmbH. All rights reserved.
引用
收藏
页码:263 / 272
页数:10
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