Assessment of paraoxonase activity and lipid peroxidation levels in diabetic and senile subjects suffering from cataract

被引：30
作者：
Hashim, Zehra [1 ]
Zarina, Shamshad [1 ]
机构：
[1] Univ Karachi, Dept Biochem, Karachi 75270, Pakistan
来源：
CLINICAL BIOCHEMISTRY | 2007年 / 40卷 / 9-10期
关键词：
paraoxonase activity; senile cataract; diabetic cataract; oxidative stress; lipid peroxidation; malondialdehyde;
D O I：
10.1016/j.clinbiochem.2007.03.015
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: The purpose of this study was to evaluate antioxidant effect of paraoxonase I activity and malondialdehyde (NIDA) levels as a marker of oxidative stress in patients suffering from cataract due to diabetes and aging. Design and methods: One hundred cataract patients (senile and diabetic) and age- and sex-matched controls were studied. Paraoxonase 1 and arylesterase activities in plasma samples were measured using paraoxon and phenylacetate as substrates, respectively. The magnitude of lipid peroxidation was established by measuring plasma MDA and oxidized low-density lipoprotein (ox LDL) levels. One-way ANOVA was employed for analysis of results. Results: We observed significantly lower plasma paraoxonase and arylesterase activities in senile and diabetic cataractous patients as compared to respective controls (p < 0.001). Plasma MDA and ox LDL levels were found to be higher in patients suffering from cataract (P < 0.001). Conclusions: The results of present study suggest that the observed decrease in PON1 activity may be due to increase in oxidative stress. It can be concluded that lower paraoxonase activity could contribute to the higher risk of cataract formation. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：705 / 709
页数：5
相关论文
共 44 条
[1]   SERUM PARAOXONASE ACTIVITY, CONCENTRATION, AND PHENOTYPE DISTRIBUTION IN DIABETES-MELLITUS AND ITS RELATIONSHIP TO SERUM-LIPIDS AND LIPOPROTEINS [J].
ABBOTT, CA ;
MACKNESS, MI ;
KUMAR, S ;
BOULTON, AJ ;
DURRINGTON, PN .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1995, 15 (11) :1812-1818
[2]   Baseline diene conjugation in LDL lipids: An indicator of circulating oxidized LDL [J].
Ahotupa, M ;
Vasankari, TJ .
FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (11-12) :1141-1150
[3]  
[Anonymous], 1999, CRC MOD NUT
[4]   Plasma catalase activity and malondialdehyde level in patients with cataract [J].
Ates, NA ;
Yildirim, Ö ;
Tamer, L ;
Ünlü, A ;
Ercan, B ;
Muslu, N ;
Kanik, A ;
Hatungil, R ;
Atik, U .
EYE, 2004, 18 (08) :785-788
[5]   Human serum paraoxonase (PON 1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants [J].
Aviram, M ;
Rosenblat, M ;
Billecke, S ;
Erogul, J ;
Sorenson, R ;
Bisgaier, CL ;
Newton, RS ;
La Du, B .
FREE RADICAL BIOLOGY AND MEDICINE, 1999, 26 (7-8) :892-904
[6]   Lipid peroxidation and cataracts: N-Acetylcarnosine as a therapeutic tool to manage age-related cataracts in human and in canine eyes [J].
Babizhayev M.A. ;
Deyev A.I. ;
Yermakova V.N. ;
Brikman I.V. ;
Bours J. .
Drugs in R & D, 2004, 5 (3) :125-139
[7]   Serum paraoxonase 1 activity and lipid peroxidation levels in patients with age-related macular degeneration [J].
Baskol, G ;
Karakucuk, S ;
Oner, AO ;
Baskol, M ;
Kocer, D ;
Mirza, E ;
Saraymen, R ;
Üstdal, M .
OPHTHALMOLOGICA, 2006, 220 (01) :12-16
[8]   Assessment of paraoxonase 1 activity and malondialdehyde levels in patients with rheumatoid arthritis [J].
Baskol, G ;
Demir, H ;
Baskol, M ;
Kilic, E ;
Ates, F ;
Kocer, D ;
Muhtaroglu, S .
CLINICAL BIOCHEMISTRY, 2005, 38 (10) :951-955
[9]   Protein oxidation in aging, disease, and oxidative stress [J].
Berlett, BS ;
Stadtman, ER .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (33) :20313-20316
[10]   ATHEROSCLEROSIS - BASIC MECHANISMS - OXIDATION, INFLAMMATION, AND GENETICS [J].
BERLINER, JA ;
NAVAB, M ;
FOGELMAN, AM ;
FRANK, JS ;
DEMER, LL ;
EDWARDS, PA ;
WATSON, AD ;
LUSIS, AJ .
CIRCULATION, 1995, 91 (09) :2488-2496
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