Cortical blood flow assessment with frequency-domain laser Doppler microscopy

被引：13
作者：
Atlan, Michael
Forget, Benoit C.
Boccara, Albert C.
Vitalis, Tania
Rancillac, Armelle
Dunn, Andrew K.
Gross, Michel
机构：
[1] Univ Paris 06, Lab Opt, ESPCI, CNRS UPR A0005, F-75005 Paris 05, France
[2] Ecole Super Phys & Chim Ind Ville Paris, Lab Neurobiol & Diversite Cellulaire, CNRS UMR 7637, F-75231 Paris 05, France
[3] Univ Texas, Dept Biomed Engn, Austin, TX 78712 USA
[4] UMPC, CNRS, ENS, UMR 8552, F-75231 Paris 05, France
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2007年 / 12卷 / 02期
关键词：
laser Doppler velocimetry; coherent optical systems; holography applications; heterodyning; interferometry; lasers in medicine;
D O I：
10.1117/1.2715184
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the assessment of cerebral blood flow (CBF) changes with a wide-field laser Doppler imager based on a CCD camera detection scheme, in vivo, in mice. The setup enables the acquisition of data in minimally invasive conditions. In contrast with conventional laser Doppler velocimeters and imagers, the Doppler signature of moving scatterers is measured in the frequency domain, by detuning a heterodyne optical detection. The quadratic mean of the measured frequency shift is used as an indicator of CBF. We observe a significant variability of this indicator in an experiment designed to induce blood flow changes. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
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页数：8
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