Flow-injection spectrophotometric determination of sulfadiazine and sulfamethoxazole in pharmaceuticals and urine

被引：20

作者：

Fan, J ^{[1
]}

Chen, YH ^{[1
]}

Feng, SL ^{[1
]}

Ye, CL ^{[1
]}

Wang, JJ ^{[1
]}

机构：

[1] Henan Normal Univ, Key Lab Environm Sci & Engn, Henan Educ Commiss, Sch Chem & Environm Sci, Xinxiang 453002, Henan, Peoples R China

来源：

ANALYTICAL SCIENCES | 2003年 / 19卷 / 03期

关键词：

D O I：

10.2116/analsci.19.419

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and sensitive flow-injection spectrophotometric method is proposed for the determination of sulfadiazine and sulfamethoxazole. This method is based on the diazotization of sulfonamide with sodium nitrite, and a coupling reaction of the diazo-compound with a-naphthylamine. The optimum experimental conditions are obtained by using the controlled and weighted centroid simplex method. The linear ranges for the determination of sulfadiazine and sulfamethoxazole are 0.2 - 20 mug ml(-1) and 0.1 - 20 mug ml(-1), and their detection limits are 0.06 mug ml(-1) and 0.05 mug ml(-1), respectively, and the sampling frequency is 130 samples per hour. The method has been used to determine sulfadiazine and sulfamethoxazole in pharmaceuticals and urine without separation. The results are in agreement with those obtained by a high-performance liquid chromatograph technique at the 95% confidence level.

引用

页码：419 / 422

页数：4

共 12 条

[1]

*CONS GEN COL OF F, 1981, EUR PHARM CONS EUR 3

[2] Determination and quantification of sulfadiazine and trimethoprim in swine tissues using liquid chromatography with ultraviolet and mass spectrometric detection [J].