Multidrug Resistance Related to Biofilm Formation in Acinetobacter baumannii and Klebsiella pneumoniae Clinical Strains from Different Pulsotypes

被引:31
作者
de Campos, Paola Amaral [1 ]
Royer, Sabrina [1 ]
da Fonseca Batistao, Deivid William [1 ]
Araujo, Bruna Fuga [1 ]
Queiroz, Licia Ludendorff [1 ]
de Brito, Cristiane Silveira [1 ]
Gontijo-Filho, Paulo P. [1 ]
Ribas, Rosineide Marques [1 ]
机构
[1] Univ Fed Uberlandia, Inst Biomed Sci, Mol Microbiol Lab, Ave Para 1720, BR-38400902 Uberlandia, MG, Brazil
关键词
PREVALENT OXA CARBAPENEMASES; PSEUDOMONAS-AERUGINOSA; MULTIPLEX PCR; GENES; MECHANISMS; OXA-143; SPREAD;
D O I
10.1007/s00284-016-0996-x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The emergence of Acinetobacter baumannii and Klebsiella pneumoniae strains in the hospital environment has been associated with the presence of multiple genetic elements, virulence factors and the ability to form biofilms. This study evaluated the biofilm formation ability of clinical and environmental A. baumannii and K. pneumoniae strains, isolated from various sources and presenting different molecular characteristics, resistance profiles and pulsed-field gel electrophoresis patterns. Fifty-three isolates were recovered from 2009 to 2014 in a Brazilian university hospital. Investigation of biofilm formation was performed for 10 strains of each species assessed by an initial adhesion assay, biofilm cell concentration and biofilm biomass, evaluated by quantitative assays in replicates, in three independent experiments. All strains of A. baumannii were able to attach to polystyrene plates, although two strains adhered to a lesser degree than the control. K. pneumoniae strains showed opposite behaviour, where only three strains adhered significantly when compared to the control. Quantitative evaluation revealed that in five A. baumannii and four K. pneumoniae isolates the biomass production could be characterised as moderate. None of the isolates were strong biofilm producers. Our results demonstrate: (1) biofilm formation is a heterogeneous property amongst A. baumannii and K. pneumoniae clinical strains and it was not associated with certain clonal types; (2) no relationship between multidrug resistance and biofilm production was observed; (3) more virulent K. pneumoniae strains tended to present higher production of biofilm.
引用
收藏
页码:617 / 627
页数:11
相关论文
共 39 条
[1]   Multi-functional analysis of Klebsiella pneumoniae fimbrial types in adherence and biofilm formation [J].
Alcantar-Curiel, Maria D. ;
Blackburn, Dana ;
Saldana, Zeus ;
Gayosso-Vazquez, Catalina ;
Iovine, Nicole ;
De la Cruz, Miguel A. ;
Giron, Jorge A. .
VIRULENCE, 2013, 4 (02) :129-138
[2]  
[Anonymous], 2011, Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement
[3]  
[Anonymous], THESIS U SAO PAULO
[4]   Genetic characterisation of drug resistance and clonal dynamics of Acinetobacter baumannii in a hospital setting in Mexico [J].
Bocanegra-Ibarias, Paola ;
Pena-Lopez, Cynthia ;
Camacho-Ortiz, Adrian ;
Llaca-Diaz, Jorge ;
Silva-Sanchez, Jesus ;
Barrios, Humberto ;
Garza-Ramos, Ulises ;
Rodriguez-Flores, Adrian Marcelo ;
Garza-Gonzalez, Elvira .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2015, 45 (03) :309-313
[5]   Dissemination of multidrug-resistant Acinetobacter baumannii genotypes carrying blaOXA-23 collected from hospitals in Rio de Janeiro, Brazil [J].
Carvalho, Karyne Rangel ;
D'Alincourt Carvalho-Assef, Ana Paula ;
Peirano, Gisele ;
Galvao dos Santos, Lia Cristina ;
Felix Pereira, Maria Jose ;
Asensi, Marise Dutra .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2009, 34 (01) :25-28
[6]  
Cassat JE, 2013, METHODS MOL BIOL, V185, P195, DOI 10.1007/978-1-62703-664-1_12
[7]   A novel PCR-based genotyping scheme for clinical Klebsiella pneumoniae [J].
Chen, Zhenhong ;
Liu, Mengying ;
Cui, Yujun ;
Wang, Li ;
Zhang, Yiquan ;
Qiu, Jingfu ;
Yang, Ruifu ;
Liu, Changting ;
Zhou, Dongsheng .
FUTURE MICROBIOLOGY, 2014, 9 (01) :21-32
[8]   Biofilms and device-associated infections [J].
Donlan, RM .
EMERGING INFECTIOUS DISEASES, 2001, 7 (02) :277-281
[9]   Biofilms: Survival mechanisms of clinically relevant microorganisms [J].
Donlan, RM ;
Costerton, JW .
CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (02) :167-+
[10]   Klebsiella pneumoniae genotype K1:: an emerging pathogen that causes septic ocular or central nervous system complications from pyogenic liver abscess [J].
Fang, Chi-Tai ;
Lai, Shau-Yan ;
Yi, Wen-Ching ;
Hsueh, Po-Ren ;
Liu, Kao-Lang ;
Chang, Shan-Chwen .
CLINICAL INFECTIOUS DISEASES, 2007, 45 (03) :284-293