Visualization of hydrogen polysulfides in living cells and in vivo via a near-infrared fluorescent probe

被引：11

作者：

Wang, Xiaoqing ^{[1
]}

Sun, Qian ^{[1
]}

Zhao, Liming ^{[2
]}

Gong, Shuwen ^{[2
]}

Xu, Li ^{[1
,3
]}

机构：

[1] Nanjing Forestry Univ, Coll Sci, Nanjing 210037, Jiangsu, Peoples R China

[2] Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Shandong, Peoples R China

[3] Nanjing Forestry Univ, Inst Mat Phys & Chem, Nanjing 210037, Jiangsu, Peoples R China

来源：

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY | 2019年 / 24卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Fluorescent sensing and imaging; Hydrogen polysulfides; BODIPY; Near-infrared; SULFIDE; SULFUR; H2S; CHEMISTRY; OXIDATION; EMISSION; BIOLOGY; BODIPY; SYSTEM; ROLES;

D O I：

10.1007/s00775-019-01718-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen polysulfides (H2Sn, n > 1), as the oxidized forms of H2S, have attracted increasing attention these years due to their involvement in signaling transduction and cytoprotective processes. It is necessary to detect H2Sn in living systems for the study of their functions. In this work, we report a BODIPY-based near-infrared emitting fluorescence probe NIR-PHS1, with "turn-on" response, rapid response rate (within 10 min), outstanding selectivity and excellent sensitivity (detection limit = 12 nM) response towards H2Sn. The probe was successfully applied to the visualizing of endogenous H2Sn in living cells. Moreover, it can be used for near-infrared in vivo imaging of H2Sn in living mice. Therefore, NIR-PHS1 could be a potential imaging tool to study the biological roles of H2Sn in living systems.

引用

页码：1077 / 1085

页数：9

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