Comparison of three molecular methods for typing Aeromonas popoffii isolates

被引：34
|
作者：
Soler, L
Figueras, MJ
Chacón, MR
Guarro, J
Martinez-Murcia, AJ
机构：
[1] Univ Rovira & Virgili, Unitat Microbiol, Dept Ciencies Med Basiques, Fac Med & Ciencies Salut, E-43201 Reus, Spain
[2] Univ Miguel Hernandez, Serv Diagnost Mol, Escuela Politecn Super, Alicante, Spain
来源：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2003年 / 83卷 / 04期
关键词：
16S-23S ISR-RFLP; Aeromonas popoffii; ERIC; REP;
D O I：
10.1023/A:1023312415276
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Three typing methods, restriction fragment length polymorphism (RFLP) of the 16S-23S intergenic spacer region (ISR), PCR amplification of the enterobacterial repetitive intergenic consensus (ERIC) and of the repetitive extragenic palindromic units (REP), were evaluated for typing 26 isolates of Aeromonas popoffii from different geographical origins. When the methods were independently studied, ERIC showed the highest discriminatory power. When the methods were combined, the best combination of two methods was ERIC with REP since strains showed a tendency to cluster according to their geographical origin. However, this tendency was reinforced with the addition of ISR-RFLP.
引用
收藏
页码：341 / 349
页数：9
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