Fish species identification in surimi-based products

被引:60
作者
Pepe, Tiziana
Trotta, Michele [1 ]
Di Marco, Isolina
Anastasio, Aniello
Bautista, Jose Manuel
Cortesi, Maria Luisa
机构
[1] Univ Naples Federico II, Dipartimento Sci Zootecn & Ispez Alimenti, Sez Ispez, Falcolta Med Vet, Naples, Italy
[2] Univ Complutense Madrid, Fac Vet, Dept Bioquim & Biol Mol4, E-28040 Madrid, Spain
关键词
surimi; Gadiformes; cytochrome b gene; PCR; direct sequencing; Theragra chalcogramma;
D O I
10.1021/jf063321o
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Whole fish morphologically identified as belonging to Theragra chalcogramma, Merluccius merluccius, Merluccius hubbsi, and Merluccius capensis and 19 fish products commercialized as surimi with different commercial brands and labeled as T. chalcogramma were analyzed by direct sequence analysis of the cytochrome b gene. A phylogenetic analysis of surimi products was performed as well. Results demonstrated that mislabeling is a large-scale phenomenon, since 84.2% of surimi-based fish products sold as T. chalcogramma (16/19) were prepared with species different from the one declared. In fact, only three samples (samples 15-17) were found to belong to T. chalcogramma. In the remaining samples, Merluccidae (samples 4-14), Gadidae (samples 18 and 19), Sparidae (sample 1), and Pomacentridae (samples 2 and 3) families were detected. A phylogenetic tree was constructed, and the bootstrap value was calculated. According to this methodology, 11 samples were grouped in the same clade as Merluccius spp.
引用
收藏
页码:3681 / 3685
页数:5
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