Transferability of Simple Sequence Repeat (SSR) Markers Developed in Red Clover (Trifolium pratense L.) to Some Trifolium Species

被引：6

作者：

Haerinasab, M. ^{[1
]}

Rahiminejad, M. R. ^{[2
]}

Ellison, N. W. ^{[3
]}

机构：

[1] Payame Noor Univ, Dept Biol, POB 19395-3697, Tehran, Iran

[2] Univ Isfahan, Dept Biol, Esfahan, Iran

[3] AgResearch, Grasslands Res Ctr, Private Bag 11008, Palmerston North, New Zealand

来源：

IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE | 2016年 / 40卷 / A1期

关键词：

Cross-species transferability; Genomic SSR; Trifolium; Red clover; MICROSATELLITE MARKERS; GENUS TRIFOLIUM; AMPLIFICATION; POLYMORPHISM; GENOME;

D O I：

10.1007/s40995-016-0011-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microsatellite markers previously developed for red clover (Trifolium pratense L.) were used in cross-amplification tests of eight other Trifolium species. Of the 20 SSR loci tested, 9 (47.37 %) had positive results and were found to be transferable to all eight species examined. The number of alleles detected at each locus ranged from 4 to 12, with an average of 7.2 in T. tumens Steven ex M. Bieb., 5.8 in T. resupinatum L., 5.1 in T. fragiferum L., 4.5 in T. tomentosum L., 3.4 in T. bullatum Boiss. & Hausskn. ex Boiss., 3.2 in T. clusii Godr. & Gren., 2.7 in T. spumosum L. and 2.1 in T. physodes Stev. ex M. B. This study has shown that SSR markers developed for red clover effectively amplify DNA from other species and this approach may be applicable for the analysis of intra-and interspecific genetic diversity of target Trifolium species.

引用

页码：59 / 62

页数：4

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