Nuclear RNA Export and Packaging Functions of HIV-1 Rev Revisited

被引:71
作者
Blissenbach, Maik [1 ]
Grewe, Bastian [1 ]
Hoffmann, Bianca [1 ]
Brandt, Sabine [1 ]
Ueberla, Klaus [1 ]
机构
[1] Ruhr Univ Bochum, Dept Mol & Med Virol, D-44780 Bochum, Germany
关键词
IMMUNODEFICIENCY-VIRUS TYPE-1; POSTTRANSCRIPTIONAL REGULATORY ELEMENT; GAG MESSENGER-RNA; CYTOPLASMIC LOCALIZATION; INDEPENDENT EXPRESSION; LENTIVIRAL VECTORS; GENE-EXPRESSION; PROTEIN; TRANSLATION; TRAFFICKING;
D O I
10.1128/JVI.02264-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Although the viral Rev protein is necessary for HIV replication, its main function in the viral replication cycle has been controversial. Reinvestigating the effect of Rev on the HIV-1 RNA distribution in various cell lines and primary cells revealed that Rev enhanced cytoplasmic levels of the unspliced HIV-1 RNA, mostly 3- to 12-fold, while encapsidation of the RNA and viral infectivity could be stimulated >1,000-fold. Although this clearly questions the general notion that the nuclear export of viral RNAs is the major function of Rev, mechanistically encapsidation seems to be linked to nuclear export, since the tethering of the nuclear export factor TAP to the HIV-1 RNA also enhanced encapsidation. Interference with the formation of an inhibitory ribonucleoprotein complex in the nucleus could lead to enhanced accessibility of the cytoplasmic HIV-1 RNA for translation and encapsidation. This might explain why Rev and tethered TAP exert the same pattern of pleiotropic effects.
引用
收藏
页码:6598 / 6604
页数:7
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