Protein expression and promoter methylation of the candidate biomarker TCF21 in gastric cancer

被引：46
作者：
Yang, Z. [1 ,2 ]
Li, D. M. [3 ]
Xie, Q. [2 ]
Dai, D. Q. [1 ,4 ]
机构：
[1] China Med Univ, Hosp 4, Ctr Canc, Shenyang 110032, Liaoning, Peoples R China
[2] China Med Univ, Hosp 4, Dept Emergency Surg, Shenyang 110032, Liaoning, Peoples R China
[3] China Med Univ, Hosp 4, Dept Anesthesiol, Shenyang 110032, Liaoning, Peoples R China
[4] China Med Univ, Hosp 4, Dept Gastrointestinal Surg, Shenyang 110032, Liaoning, Peoples R China
来源：
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | 2015年 / 141卷 / 02期
关键词：
TCF21; Gastric cancer; Gene expression; DNA methylation; Epithelial-to-mesenchymal transition; EPITHELIAL-MESENCHYMAL TRANSITION; LUNG; GENE; METASTASIS; HEAD; CAPSULIN; DEFINES; KISS1;
D O I：
10.1007/s00432-014-1809-x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Transcription factor 21 (TCF21) has been identified as a candidate tumor suppressor at 6q23-q24 that is epigenetically inactivated in many types of human cancers. This study aimed to determine the expression of TCF21 mRNA and protein in gastric cancer cell lines and tissue specimens and then investigate the prognostic impact of TCF21 expression in gastric cancer and analyze the relationship between TCF21 expression and methylation level. We used real-time PCR and immunohistochemical staining to detect the expression of TCF21 and used methylation-specific-PCR to determine the methylation status of TCF21 in gastric cancer samples and gastric cancer cell lines. The results showed that TCF21 expression level in gastric cancer samples was significantly lower than in normal adjacent tissue samples. The Kaplan-Meier survival analysis demonstrated that TCF21 was a significant prognosticator of cancer-specific survival (p = 0.001). Furthermore, the methylation level of TCF21 in gastric cancer samples was much higher than the samples in normal adjacent tissue. Treatment with the DNA methyltransferase inhibitor 5-Aza-2'-deoxy-cytidine can upregulate the expression of TCF21 in gastric cancer cells. These results suggest that the low expression of TCF21 was an independent prognostic factor for poor survival in patients with gastric cancer. Aberrant methylation was an important reason for the downregulation of TCF21 and may be associated with tumorigenesis in gastric cancer.
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页码：211 / 220
页数：10
相关论文
共 34 条
  • [1] Identification of a panel of sensitive and specific DNA methylation markers for squamous cell lung cancer
    Anglim, Paul P.
    Galler, Janice S.
    Koss, Michael N.
    Hagen, Jeffrey A.
    Turla, Sally
    Campan, Mihaela
    Weisenberger, Daniel J.
    Laird, Peter W.
    Siegmund, Kimberly D.
    Laird-Offringa, Ite A.
    [J]. MOLECULAR CANCER, 2008, 7 (1)
  • [2] [Anonymous], 2020, CA Cancer J Clin, DOI DOI 10.3322/CAAC.21590
  • [3] Epigenetic deregulation of TCF21 inhibits metastasis suppressor KISS1 in metastatic melanoma
    Arab, Khelifa
    Smith, Laura T.
    Gast, Andreas
    Weichenhan, Dieter
    Huang, Joseph Po-Hsien
    Claus, Rainer
    Hielscher, Thomas
    Espinosa, Allan V.
    Ringel, Matthew D.
    Morrison, Carl D.
    Schadendorf, Dirk
    Kumar, Rajiv
    Plass, Christoph
    [J]. CARCINOGENESIS, 2011, 32 (10) : 1467 - 1473
  • [4] Transitions between epithelial and mesenchymal states in development and disease
    Baum, Buzz
    Settleman, Jeffrey
    Quinlan, Margaret P.
    [J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2008, 19 (03) : 294 - 308
  • [5] Basic Helix-Loop-Helix Transcription Factor TCF21 Is a Downstream Target of the Male Sex Determining Gene SRY
    Bhandari, Ramji K.
    Sadler-Riggleman, Ingrid
    Clement, Tracy M.
    Skinner, Michael K.
    [J]. PLOS ONE, 2011, 6 (05):
  • [6] TCF21 and PCDH17 methylation An innovative panel of biomarkers for a simultaneous detection of urological cancers
    Costa, Vera L.
    Henrique, Rui
    Danielsen, Stine A.
    Eknaes, Mette
    Patrcio, Patricia
    Morais, Antonio
    Oliveira, Jorge
    Lothe, Ragnhild A.
    Teixeira, Manuel R.
    Lind, Guro E.
    Jeronimo, Carmen
    [J]. EPIGENETICS, 2011, 6 (09) : 1120 - 1130
  • [7] Epidemiology of gastric cancer
    Crew, Katherine D.
    Neugut, Alfred I.
    [J]. WORLD JOURNAL OF GASTROENTEROLOGY, 2006, 12 (03) : 354 - 362
  • [8] DNA methylation in development and human disease
    Gopalakrishnan, Suhasni
    Van Emburgh, Beth O.
    Robertson, Keith D.
    [J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2008, 647 (1-2) : 30 - 38
  • [9] miRBase: microRNA sequences, targets and gene nomenclature
    Griffiths-Jones, Sam
    Grocock, Russell J.
    van Dongen, Stijn
    Bateman, Alex
    Enright, Anton J.
    [J]. NUCLEIC ACIDS RESEARCH, 2006, 34 : D140 - D144
  • [10] The role of epithelial-mesenchymal transition in cancer pathology
    Guarino, Marcello
    Rubino, Barbara
    Ballabio, Gianmario
    [J]. PATHOLOGY, 2007, 39 (03) : 305 - 318
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