The formation of a quaternary structure by recombinant analogs of spider silk proteins

被引：7

作者：

Sokolova, O. S. ^{[1
]}

Bogush, V. G. ^{[2
]}

Davydova, L. I. ^{[2
]}

Polevova, S. V. ^{[1
]}

Antonov, S. A. ^{[1
]}

Neretina, T. V. ^{[3
]}

Klinov, D. V. ^{[3
]}

Debabov, V. G. ^{[2
]}

Kirpichnikov, M. P. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Biol, Moscow 119991, Russia

[2] State Res Ctr GosNIIGenet, Moscow 113545, Russia

[3] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

MOLECULAR BIOLOGY | 2010年 / 44卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

recombinant proteins; spidroins; scanning electron microscopy; transmission electron microscopy; microfibers; DRAGLINE SILK; DESIGN; FIBERS;

D O I：

10.1134/S0026893310010188

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The morphology of the fibers formed by recombinant analogs of dragline spider silk proteins, spidroins 1 and 2, was studied. It has been shown that the extension of the initial fiber, the so-called as-spun fiber, leads to remodeling of the spongy matrix with the formation of microfibers, which is accompanied by a decrease in the fiber diameter. The breaking strength of the fiber depends not only on the primary structure of the constituent protein, but also on the way it was formed. Simulation of the assembly of microfibers and the fibers formed of them can clarify the natural spider web spinning and enhance the development of technology for producing biomaterials with unique properties.

引用

页码：150 / 157

页数：8

共 50 条

[1] The formation of a quaternary structure by recombinant analogs of spider silk proteins
O. S. Sokolova
V. G. Bogush
L. I. Davydova
S. V. Polevova
S. A. Antonov
T. V. Neretina
D. V. Klinov
V. G. Debabov
M. P. Kirpichnikov
Molecular Biology, 2010, 44 : 150 - 157
[2] Recombinant DNA production of spider silk proteins
Tokareva, Olena
Michalczechen-Lacerda, Valquiria A.
Rech, Elibio L.
Kaplan, David L.
MICROBIAL BIOTECHNOLOGY, 2013, 6 (06): : 651 - 663
[3] Recombinant Production of Spider Silk Proteins
Heidebrecht, Aniela
Scheibel, Thomas
ADVANCES IN APPLIED MICROBIOLOGY, VOL 82, 2013, 82 : 115 - 153
[4] A Novel Model System for Design of Biomaterials Based on Recombinant Analogs of Spider Silk Proteins
Vladimir G. Bogush
Olga S. Sokolova
Lyubov I. Davydova
Dmitri V. Klinov
Konstantin V. Sidoruk
Natalya G. Esipova
Tatyana V. Neretina
Igor A. Orchanskyi
Vsevolod Yu Makeev
Vladimir G. Tumanyan
Konstantin V. Shaitan
Vladimir G. Debabov
Mikhail P. Kirpichnikov
Journal of Neuroimmune Pharmacology, 2009, 4
[5] A Novel Model System for Design of Biomaterials Based on Recombinant Analogs of Spider Silk Proteins
Bogush, Vladimir G.
Sokolova, Olga S.
Davydova, Lyubov I.
Klinov, Dmitri V.
Sidoruk, Konstantin V.
Esipova, Natalya G.
Neretina, Tatyana V.
Orchanskyi, Igor A.
Makeev, Vsevolod Yu
Tumanyan, Vladimir G.
Shaitan, Konstantin V.
Debabov, Vladimir G.
Kirpichnikov, Mikhail P.
JOURNAL OF NEUROIMMUNE PHARMACOLOGY, 2009, 4 (01) : 17 - 27
[6] Recombinant Spider Silk Proteins for Applications in Biomaterials
Spiess, Kristina
Lammel, Andreas
Scheibel, Thomas
MACROMOLECULAR BIOSCIENCE, 2010, 10 (09) : 998 - 1007
[7] Host Systems for the Production of Recombinant Spider Silk
Whittall, Dominic R.
Baker, Katherine, V
Breitling, Rainer
Takano, Eriko
TRENDS IN BIOTECHNOLOGY, 2021, 39 (06) : 560 - 573
[8] Suitable electrospinning approaches for recombinant spider silk proteins
Sommer, Christoph
Scheibel, Thomas
OPEN CERAMICS, 2023, 15
[9] Structure and pH-induced alterations of recombinant and natural spider silk proteins in solution
Leclerc, Jeremie
Lefevre, Thierry
Pottier, Fabien
Morency, Louis-Philippe
Lapointe-Verreault, Camille
Gagne, Stephane M.
Auger, Michele
BIOPOLYMERS, 2012, 97 (06) : 337 - 346
[10] Controlled Hydrogel Formation of a Recombinant Spider Silk Protein
Schacht, Kristin
Scheibel, Thomas
BIOMACROMOLECULES, 2011, 12 (07) : 2488 - 2495

← 1 2 3 4 5 →