Genetic typing of the mouse and rat nude mutations by PCR and restriction enzyme analysis

被引：14

作者：

Hirasawa, T ^{[1
]}

Yamashita, H ^{[1
]}

Makino, S ^{[1
]}

机构：

[1] Shionogi & Co Ltd, Ctr Expt Anim Dev, Shiga 52034, Japan

来源：

EXPERIMENTAL ANIMALS | 1998年 / 47卷 / 01期

关键词：

genotyping; nude mutation; PCR-RFLP;

D O I：

10.1538/expanim.47.63

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A genetic typing method for the mouse and rat nude mutations by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Since restriction sites useful for RFLP analysis do not exist in the mouse nu and rat rnu mutations, artificial restriction sites were introduced by PCR with modified primers. Three genotypes in the mouse (nu/nu, nu/+ and +/+) or rat (rnu/rnu, rnu/+ and +/+) are rapidly differentiated with the PCR-RFLP assay. In addition, congenic nude strains can be efficiently established by using this assay. Finally, genetic mapping of the rnu locus was performed with microsatellite markers. The locus order on rat chromosome 10 was D10Mgh14-(2.0cM)-D10Mit2-(1.4cM)-rnu-(0.7cM)-D10Mgh6-(2.7cM)-D10Mit8.

引用

页码：63 / 67

页数：5

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