Simple spectrofluorimetric methods for determination of veterinary antibiotic drug (apramycin sulfate) in pharmaceutical preparations and milk samples

被引:8
作者
Mabrouk, Mokhtar M. [1 ]
Noureldin, Hind A. M. [2 ]
Badr, Ibrahim H. A. [3 ]
Saad, Amira H. K. [1 ]
机构
[1] Tanta Univ, Dept Analyt Chem, Fac Pharm, Tanta, Egypt
[2] Badr Univ Cairo, Dept Analyt Chem, Fac Pharm, Cairo, Egypt
[3] Ain Shams Univ, Dept Chem, Fac Sci, Cairo, Egypt
关键词
Apramycin sulfate; Spectrofluorimetric; Micelle-enhancement; Milk; LIQUID-CHROMATOGRAPHY; AMINOGLYCOSIDE ANTIBIOTICS; DERIVATIZATION; PERFORMANCE; RESIDUES; TISSUES; FORMS; ASSAY;
D O I
10.1016/j.saa.2019.117395
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
This work describes the development of highly sensitive as well as simple two spectrofluorimetric methods for the determination of apramycin sulfate. The first method depends on measuring the inherent native fluorescence of the aqueous neutral solution of the drug at 388 nm (lambda(ex) 335 nm). While the second method mainly based on enhancing the native fluorescence intensity of the drug using sodium dodecyl sulfate (SDS) micellar media by about 4 fold enhancement. The fluorescence intensity - concentration relationship for the two methods was found rectilinear over the concentration range 1.0 -100.0 and 0.1-20.0 mu g/mL for the first and second method respectively. The limit of detection for method I and II were 0.05 and 0.02 mu g/mL respectively. The proposed methods can be effectively connected for the assurance of the medication without impedances from common normal excipients. Furthermore, the two methods were high sensitive enough for the assurance of the drug in spiked milk samples with high percentage recoveries. (c) 2019 Elsevier B.V. All rights reserved.
引用
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页数:5
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共 30 条
  • [1] Determination of the anti-viral drug Ribavirin in dosage forms via micelle-enhanced spectrofluorimetric method
    Alarfaj, Nawal A.
    El-Tohamy, Maha F.
    [J]. LUMINESCENCE, 2013, 28 (02) : 190 - 194
  • [2] Antunes EDB, 2011, BRAZ J PHARM SCI, V47, P261
  • [3] Micelle-enhanced spectrofluorimetric method for determination of lacidipine in tablet form; application to content uniformity testing
    Belal, F.
    El-Din, M. Sharaf
    Tolba, M. M.
    Alaa, H.
    [J]. LUMINESCENCE, 2015, 30 (06) : 805 - 811
  • [4] Simple confirmatory assay for analyzing residues of aminoglycoside antibiotics in bovine milk:: hot water extraction followed by liquid chromatography-tandem mass spectrometry
    Bogialli, S
    Curini, R
    Di Corcia, A
    Laganà, A
    Mele, M
    Nazzari, M
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2005, 1067 (1-2) : 93 - 100
  • [5] BONTCHEV PR, 1984, MIKROCHIM ACTA, V3, P459
  • [6] Immunochemical detection of apramycin as a contaminant in tissues of edible animals
    Burkin, Maksim
    Galvidis, Inna
    [J]. FOOD CONTROL, 2013, 34 (02) : 408 - 413
  • [7] Comparative study on the analytical performance of three waveforms for the determination of several aminoglycoside antibiotics with high performance liquid chromatography using amperometric detection
    Cai, YQ
    Cai, W
    Cheng, J
    Mou, SF
    Lu, YQ
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2005, 1085 (01) : 124 - 130
  • [8] Postantibiotic and physiological effects of tilmicosin, tylosin, and apramycin at subminimal and suprainhibitory concentrations on some swine and bovine respiratory tract pathogens
    Diarra, MS
    Malouin, F
    Jacques, M
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 1999, 12 (03) : 229 - 237
  • [9] Pharmacokinetics of gentamicin and apramycin in turkeys roosters and hens in the context of pharmacokinetic-pharmacodynamic relationships
    Haritova, AM
    Djeneva, HA
    Lashev, LD
    Sotirova, PG
    Gurov, BI
    Dyankov, VN
    [J]. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 2004, 27 (05) : 381 - 384
  • [10] Higgins C E, 1967, Antimicrob Agents Chemother (Bethesda), V7, P324