This work describes the development of highly sensitive as well as simple two spectrofluorimetric methods for the determination of apramycin sulfate. The first method depends on measuring the inherent native fluorescence of the aqueous neutral solution of the drug at 388 nm (lambda(ex) 335 nm). While the second method mainly based on enhancing the native fluorescence intensity of the drug using sodium dodecyl sulfate (SDS) micellar media by about 4 fold enhancement. The fluorescence intensity - concentration relationship for the two methods was found rectilinear over the concentration range 1.0 -100.0 and 0.1-20.0 mu g/mL for the first and second method respectively. The limit of detection for method I and II were 0.05 and 0.02 mu g/mL respectively. The proposed methods can be effectively connected for the assurance of the medication without impedances from common normal excipients. Furthermore, the two methods were high sensitive enough for the assurance of the drug in spiked milk samples with high percentage recoveries. (c) 2019 Elsevier B.V. All rights reserved.
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Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, BulgariaTrakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Haritova, AM
Djeneva, HA
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机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Djeneva, HA
Lashev, LD
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机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Lashev, LD
Sotirova, PG
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机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Sotirova, PG
Gurov, BI
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机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Gurov, BI
Dyankov, VN
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机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
机构:
Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, BulgariaTrakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Haritova, AM
Djeneva, HA
论文数: 0引用数: 0
h-index: 0
机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Djeneva, HA
Lashev, LD
论文数: 0引用数: 0
h-index: 0
机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Lashev, LD
Sotirova, PG
论文数: 0引用数: 0
h-index: 0
机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Sotirova, PG
Gurov, BI
论文数: 0引用数: 0
h-index: 0
机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria
Gurov, BI
Dyankov, VN
论文数: 0引用数: 0
h-index: 0
机构:Trakia Univ, Fac Vet Med, Dept Pharmacol Toxicol & Therapeut, Stara Zagora 6000, Bulgaria