The single and joint toxicity effects of chlorpyrifos and beta-cypermethrin in zebrafish (Danio rerio) early life stages

被引:83
作者
Zhang, Jiayu [1 ]
Liu, Lili [1 ]
Ren, Lei [1 ]
Feng, Weimin [1 ]
Lv, Peng [1 ]
Wu, Wei [1 ]
Yan, Yanchun [1 ]
机构
[1] Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
关键词
Pesticides; Zebrafish embryo; Developmental toxicity; Oxidative stress; RNA-Seq; OXIDATIVE STRESS; RISK-ASSESSMENT; PESTICIDE-RESIDUES; CELL-CYCLE; WATER; INSECTICIDES; EXPOSURE; IMMUNOTOXICITY; POLLUTION; APOPTOSIS;
D O I
10.1016/j.jhazmat.2017.03.055
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Water environment pollution caused by the widespread application of beta-cypermethrin (BCP) and chlorpyrifos (CPF) in agriculture has attracted extensive concern of the world. In this study, zebrafish was used as a model to investigate the individual and joint toxicity of BCP and CPF. In the acute toxicity test, 3 hpf embryos were exposed to various concentrations of CPF, BCP and their binary mixtures (MIX) for 96 h. The results indicated that these two pesticides and mixtures induced malformation and death in larvae, and affected hatchability. These two pesticides in mixtures were verified to act together in a synergistic manner under experimental conditions. Oxidative stress assaying manifested that CPF, BCP and MIX altered CAT, SOD and GST activities and MDA content, resulting in oxidative damage in larvae. By pathology analysis, CPF (236 mu g/L), BCP (5.9 mu g/L) and MIX (236 mu g/L CPF + 5.9 mu g/L BCP) were found to trigger liver lesions and promote apoptosis in tissues. The transcriptome sequencing suggested that ECM-receptor interaction, focal adhesion, cell cycle, DNA replication, phototransduction and adherens junction pathways were closely associated with the toxicity of these two pesticides. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:121 / 131
页数:11
相关论文
共 50 条
[41]   Single and combined acute and subchronic toxic effects of microplastics and copper in zebrafish (Danio rerio) early life stages [J].
Santos, Dercia ;
Felix, Luis ;
Luzio, Ana ;
Parra, Susana ;
Bellas, Juan ;
Monteiro, Sandra M. .
CHEMOSPHERE, 2021, 277
[42]   Toxicity effects in zebrafish embryos (Danio rerio) induced by prothioconazole [J].
Tian, Sinuo ;
Teng, Miaomiao ;
Meng, Zhiyuan ;
Yan, Sen ;
Jia, Ming ;
Li, Ruisheng ;
Liu, Li ;
Yan, Jin ;
Zhou, Zhiqiang ;
Zhu, Wentao .
ENVIRONMENTAL POLLUTION, 2019, 255
[43]   Effects of metal nanoparticles on the lateral line system and behaviour in early life stages of zebrafish (Danio rerio) [J].
McNeil, Paul L. ;
Boyle, David ;
Henry, Theodore B. ;
Handy, Richard D. ;
Sloman, Katherine A. .
AQUATIC TOXICOLOGY, 2014, 152 :318-323
[44]   Toxic effects and mechanisms of common pesticides (Roundup and chlorpyrifos) and their mixtures in a zebrafish model (Danio rerio) [J].
Falfushynska, Halina ;
Khatib, Ihab ;
Kasianchuk, Nadiia ;
Lushchak, Oleg ;
Horyn, Oksana ;
Sokolova, Inna M. .
SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 833
[45]   Embryotoxicity of methylparaben to zebrafish (Danio rerio) early-life stages [J].
Merola, C. ;
Perugini, M. ;
Conte, A. ;
Angelozzi, G. ;
Bozzelli, M. ;
Amorena, M. .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2020, 236
[46]   Effects of gamma radiation on the early developmental stages of Zebrafish (Dario rerio) [J].
Kumar, M. K. Praveen ;
Shyama, S. K. ;
Kashif, Shamim ;
Dubey, S. K. ;
Avelyno, D'costa ;
Sonaye, B. H. ;
Samit, B. Kadam ;
Chaubey, R. C. .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2017, 142 :95-101
[47]   Alleviative effects of quercetin of Botrytis cinerea-induced toxicity in zebrafish (Danio rerio) larvae [J].
Shi, Shengnan ;
Wang, Ju ;
Liu, Changhong ;
Zheng, Lei .
FISH & SHELLFISH IMMUNOLOGY, 2023, 142
[48]   Single and combined effects of carbamazepine and copper on nervous and antioxidant systems of zebrafish (Danio rerio) [J].
Jia, Dantong ;
Li, Xiuwen ;
Du, Sen ;
Xu, Ning ;
Zhang, Wenming ;
Yang, Ruyi ;
Zhang, Yunhai ;
He, Yide ;
Zhang, Yongjun .
ENVIRONMENTAL TOXICOLOGY, 2020, 35 (10) :1091-1099
[49]   Impacts of isopyrazam exposure on the development of early-life zebrafish (Danio rerio) [J].
Hongzhou Yao ;
Xiao Xu ;
Ying Zhou ;
Chao Xu .
Environmental Science and Pollution Research, 2018, 25 :23799-23808
[50]   Developmental toxicity, immunotoxicity and cardiotoxicity induced by methidathion in early life stages of zebrafish [J].
Wu, Yuanzhao ;
Chen, Tianyi ;
Xia, Yumei ;
Wang, Jiawen ;
Wang, Anli ;
Wang, Binjie ;
Wang, Jiye ;
Yao, Weixuan .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2023, 194