Contrast media augmented apoptosis of cultured renal mesangial, tubular, epithelial, endothelial, and hepatic cells

被引:36
|
作者
Peer, A
Averbukh, Z [1 ]
Berman, S
Modai, D
Averbukh, M
Weissgarten, J
机构
[1] Assaf Harofeh Med Ctr, Div Nephrol, IL-70300 Zerifin, Israel
[2] Assaf Harofeh Med Ctr, Dept Intervent Radiol, IL-70300 Zerifin, Israel
[3] Tel Aviv Univ, Sackler Fac Med, Tel Aviv, Israel
关键词
apoptosis; mesangium; renal endothelium/epithelium; hepatic cells; contrast media; RADIOCONTRAST AGENTS; RAT-KIDNEY; COMPARATIVE CYTOTOXICITY; DNA FRAGMENTATION; IN-VITRO; IOMEPROL; INDOMETHACIN; ANGIOGRAPHY; DIATRIZOATE; MONOLAYERS;
D O I
10.1097/00004424-200303000-00007
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
RATIONALE AND OBJECTIVE. Nephrotoxicity of contrast media, resulting in apoptosis and acute necrosis of tubular cells, is well documented. No studies concerning mesangial cells apoptosis have been published yet. AIM: Apoptosis of cultured mesangial, tubular, and hepatic cell lines was investigated following exposure to different contrast media. METHODS. Apoptosis was assessed by TUNEL assay and verified by Mayer Hematoxylin staining. RESULTS. lopromide, Ioxaglate, and Ioxatalamate induced apoptosis in all cell cultures at final concentrations ranged from 0.1% to 10.0%. However, only 1% to 10% Iomeprol elicited a significant apoptosis. Moreover, at 10% concentration, Iomeprol induced significantly less apoptosis than Iopromide, Ioxaglate, or Ioxatalamate. CONCLUSIONS. First, Iomeprol, which has a different physico-chemical properties, proved to be less proapoptotic compared with other contrast compounds. Second, all types of cells similarly respond by apoptosis to contrast media induced injury. However, apoptosis of mesangial cells might generate additional deleterious effects in vivo.
引用
收藏
页码:177 / 182
页数:6
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