An alternative pathway for plant in vitro regeneration of insecticidal tree Melia azedarach L. derived from the induction of somatic embryogenesis

被引：1

作者：

Sharry, S. ^{[1
]}

Abedini, W. ^{[1
]}

Lede, S. ^{[1
]}

Ponce, J. L. Cabrera ^{[2
]}

Estrella, L. Herrera ^{[2
]}

Cano, R. M. Rangel ^{[2
]}

机构：

[1] Univ Nacl La Plata, Fac Ciencias Agr & Forestales, CE Pro Ve, CC 31, RA-1900 La Plata, Buenos Aires, Argentina

[2] CINVESTAV IPN, Dept Genetica, Unidad Irapuato, Buenos Aires, Argentina

来源：

PROCEEDINGS OF THE VTH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURE BREEDING, VOLS 1 AND 2 | 2006年 / 725期

关键词：

Melia azedarach; somatic embryogenesis; direct and indirect morphogenesis; plant regeneration; adenine;

D O I：

10.17660/ActaHortic.2006.725.70

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A model for highly efficient method for plant regeneration from somatic embryos of chinaberry tree (Melia azedarach L.) was obtained. Mature seeds were cultured on Murashige and Skoog's medium supplemented with NAA/BA to induce embryogenic tissues. Embryo proliferation occurred with high level citokinin concentration (10 mg/L BAP) and germination occurred on free hormone medium or in BAP/AG3 medium. Plants were regenerated from both, direct embryo formation and through an intermediary callus phase. Results showed 100% embryo conversion to plant. Regenerated plants were normal phenotypically and continued to grow after transfer to a greenhouse environment with 100% of surviving with high rate of growth.

引用

页码：489 / +

页数：4

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