Screening of Some Tanzanian Medicinal Plants for their Trypanocidal and Cytotoxic Activities

The objective of the present study was to evaluate in vitro antitrypanosomal and cytotoxic activities of crude extracts of 20 traditionally used medicinal plants of Tanzania. A total of 40 extracts (dichloromethane and methanol) were screened for antiproliferative activity of bloodstream form of T. b. brucei and human leukaemia HL-60 cell. Inhibition of cell proliferation was assessed using resazurin as vital stain. Of the 40 extracts tested, the dichloromethane extract from bark of Warburgia salutaris (Canellaceae) exhibited the most potent antitrypanosomal activity with an IC50 value of 10.68 mu g/ml. A dichloromethane extract from Lannea stuhlmannii (Anacardiaceae) was found to be the most cytotoxic extract against HL-60 (IC50 = 27.15 mu g/ml). Out of the 20 plants tested, 5 plants exhibited trypanocidal activity with IC50 values below 20 mu g/ml. These 5 plants: Entandrophragma bussei (Meliaceae), Securidaca longepedunculata (Polygalaceae), Warburgia salutaris (Canellaceae), Zanha africana (Sapindaceae) and Zantharylum chalybeum (Rutaceae) could therefore serve as sources of lead compounds for treatment of trypanosomiasis. Copyright (C) 2009 John Wiley & Sons, Ltd.