Ultrasound-mediated gene delivery: Influence of contrast agent on transfection

被引：64
作者：
Mehier-Humbert, Sophie
Yan, Feng
Frinking, Peter
Schneider, Michel
Guy, Richard H.
Bettinger, Thierry
机构：
[1] Bracco Res SA, CH-1228 Plan Les Ouates, Switzerland
[2] Univ Geneva, Dept Pharm & Biopharm, CH-1211 Geneva 4, Switzerland
[3] Univ Bath, Sch Pharm & Pharmacol, Bath BA2 7AY, Avon, England
来源：
BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 03期
关键词：
D O I：
10.1021/bc0602432
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MAT B III cells were insonified at ultrasound frequencies of 1.15 and 2.25 MHz in the presence of different ultrasound contrast agents (UCA) and a plasmid encoding for the green fluorescent protein. The transfection efficiency was assessed by flow cytometry, while contrast agent destruction by ultrasound was evaluated using optical and scanning electronic microscopies. It was found that the gas and shell properties of the UCA have an important influence on cell transfection. A good correlation was observed between bubble destruction and transfection rate, and it was demonstrated, for the first time, that hard-shelled contrast agents (gas microcapsules) are promising candidates for ultrasound-mediated gene delivery.
引用
收藏
页码：652 / 662
页数：11
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