Diploid spermatozoa caused by failure of the second meiotic division in a bull

被引：7

作者：

Revay, T. ^{[2
]}

Kopp, C. ^{[1
]}

Flyckt, A. ^{[1
]}

Taponen, J. ^{[1
]}

Ijas, R. ^{[3
]}

Nagy, S. ^{[4
]}

Kovacs, A. ^{[5
]}

Rens, W. ^{[6
]}

Rath, D. ^{[7
]}

Hidas, A. ^{[2
]}

Taylor, J. F. ^{[8
]}

Andersson, M. ^{[1
]}

机构：

[1] Univ Helsinki, Dept Prod Anim Med, Saarentaus, Finland

[2] Res Inst Anim Breeding & Nutr, Godollo, Hungary

[3] Breeding Serv Cooperat, Pieksamaki, Finland

[4] Univ Pannonia, Inst Analyt Environm Sci & Limnol, Veszprem, Hungary

[5] Univ Debrecen, Inst Anim Breeding Sci, Ctr Agr Sci & Engn, Debrecen, Hungary

[6] Univ Cambridge, Dept Vet Med, Cambridge, England

[7] Inst Anim Breeding, Fed Agr Res Ctr, Neustadt, Germany

[8] Univ Missouri, Dept Anim Sci, Columbia, MO USA

来源：

THERIOGENOLOGY | 2010年 / 73卷 / 04期

关键词：

Bull sperm; Diploid spermatozoa; Flow cytometry and sorting; Fluorescence in situ hybridization; Macrocephalic spermatozoa; Second meiotic division; Y-chromosome and X-chromosome; IN-SITU HYBRIDIZATION; INTRACYTOPLASMIC SPERM INJECTION; POLYPLOID SPERMATOZOA; CATTLE; FISH; FERTILITY; MUTATION; MEIOSIS; DEFECTS; SEMEN;

D O I：

10.1016/j.theriogenology.2009.09.025

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An artificial insemination bull (Bos taurus) exhibiting 23% macrocephalic spermatozoa in the ejaculate was investigated. Spermatozoa with a projected head area of >= 52 mu m(2) were considered macrocephalic. Diploidy was assumed from the measurement of sperm head area and proved by flow cytometry, which was used to sort the sperm into haploid and diploid fractions. Fluorescence in situ hybridization was used to detect the sex chromosomes with an X-Y probe set. Diploid spermatozoa most likely originate from a defective second meiotic division (M2 diploids), as only 0.7% XY-bearing spermatozoa (M1 diploids) were detected in the spermatozoa of the flow cytometric diploid sort. The painting probes generated a single X or Y spot for both unsorted semen and diploid sorted spermatozoa. This indicates a close proximity of the nonpartitioned sister chromatids in the spermatozoa. The BC1.2 probe, which labels BTAYp13-12, was used to clarify the presence of the two chromatids in the singular signal of the simultaneously hybridized Y-painting probe. In scoring more than 1000 randomly sampled spermatozoa hybridized with the BC1.2 probe, 32% showed the YY diploid signal and 18% the Y signal. The sperm diploidy in this bull was caused by an incomplete partitioning of sister chromatids during the second meiotic division (M2) associated with a failure in nuclear cleavage. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：421 / 428

页数：8

共 27 条

[21] An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures [J].