Ultrasensitive Detection of ctDNA by Target-Mediated In Situ Growth of DNA Three-Way Junction on the Electrode

被引：7
作者：
Chen, Xifeng [1 ,2 ]
Tang, Yuguo [1 ,2 ]
Yan, Ruhong [3 ]
Miao, Peng [1 ,2 ]
机构：
[1] Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
[2] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
[3] Nanjing Med Univ, Affiliated Suzhou Sci & Technol Town Hosp, Dept Clin Lab, Suzhou 215153, Peoples R China
来源：
CHEMELECTROCHEM | 2020年 / 7卷 / 01期
关键词：
three-way junction; DNA; strand displacement; target recycling; biosensors; LABEL-FREE; ELECTROCHEMICAL DETECTION; SENSOR; SURFACE; PLASMA;
D O I：
10.1002/celc.201901657
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Circulation tumor DNA (ctDNA) is an emerging biomarker for diagnosis and therapy of cancers. Herein, an ultrasensitive electrochemical method for ctDNA quantification is developed by in situ growth of DNA three-way junction on the electrode. Three hairpin-structured DNA probes are used as fuel strands and strand-displacement polymerization reactions are initiated by target ctDNA for the in situ growth of the DNA junction structure, which changes the electron transfer pathways of electrochemical species. By further employing a reductant-mediated amplification, the method shows sub-femtomolar sensitivity. Meanwhile, specific analysis of ctDNA is achieved with negligible interference by DNAs with one or more mismatched sites. Therefore, the proposed method may have great analytical and clinical applications.
引用
收藏
页码：64 / 68
页数：5
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