Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)

被引：0

作者：

Chaichanathong, Sirinart ^{[1
,2
]}

Wajjwalku, Worawidh ^{[3
]}

Prasanwong, Chayanid ^{[4
]}

Suksamranthaweerat, Melanee ^{[4
]}

Rasitanon, Piyawan ^{[4
]}

Phojun, Aunchisa ^{[4
]}

Watanangura, Antja ^{[4
]}

Boodde, Orawan ^{[5
]}

Thongtip, Nikorn ^{[1
,2
,6
]}

机构：

[1] Kasetsart Univ, Ctr Agr Biotechnol, Nakhon Pathom 73140, Thailand

[2] Ctr Excellence Agr Biotechnol AG BIO PERDO CHE, Bangkok 10900, Thailand

[3] Kasetsart Univ, Fac Vet Med, Dept Pathol, Nakhon Pathom 73140, Thailand

[4] Kasetsart Univ, Fac Vet Med, Bangkok 10900, Thailand

[5] Kasetsart Univ, Fac Vet Med, Dept Farm Resources & Prod Med, Nakhon Pathom 73140, Thailand

[6] Kasetsart Univ, Fac Vet Med, Dept Large Anim & Wildlife Clin Sci, Nakhon Pathom 73140, Thailand

来源：

THAI JOURNAL OF VETERINARY MEDICINE | 2014年 / 44卷 / 04期

关键词：

cervidae; diagnosis; ELISA; tuberculosis; vaccine; MYCOBACTERIUM-BOVIS INFECTION; ODOCOILEUS-VIRGINIANUS; CERVUS-ELAPHUS; TUBERCULOSIS; EXPOSURE; MPB70; ELK;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccination has been studied in cervids. In this study 15 rusa deer were tested for responsiveness to mycobacterial reaction using a comparative cervical skin test (CCT). The rusa deer were divided into 2 groups; BCG vaccination group, which were inoculated with a single dose of 2x10(6) cfu M. bovis BCG Tokyo subcutaneously (n=8) and control group (n=7). The MPB83 protein was constructed and used as a specific antigen for an indirect ELISA to determine antibody levels against BCG vaccination in rusa deer. Sera were collected pre-vaccination and 4, 10, 15, 20 and 25 weeks post-vaccination. The antibody levels at each time point were determined by indirect ELISA using the MPB83 antigen. In the vaccination group, mean difference in optical density (ODs) increased significantly (p<0.05) 4 weeks after vaccination. Thereafter, the antibody level continuously declined until the final measurement at 25 weeks. The antibody levels against the MPB83 protein in the vaccination group were higher than in the control group at 4, 10, 15 and 20 weeks post-vaccination and were not different between the positive and negative CCT animals. In the control group, the mean difference in ODs were not significantly different at any measured time point. In conclusion, the immunity response to BCG-vaccination in the rusa deer could be monitored by indirect ELISA using the specific antigen MPB83. Therefore, further studies to determine protective level of antibody against BCG and cell-mediated immune responses should be implemented.

引用

页码：407 / 413

页数：7